Characterization of principal compositions in the roots of Angelica sinensis by HPLC-ESI-MSn and chemical comparison of its different parts

doi:10.5246/jcps.2014.06.053

Abstract

Abstract:

Angelicae Sinensis Radix, Danggui in Chinese, is an important traditional Chinese medicine derived from the roots of Angelica sinensis (Oliv.) Diels. HPLC-ESI-MSⁿ was applied to analyze the chemical constituents in the methanol extract of Danggui. A total of 24 compounds were identified or tentatively characterized based on their UV and mass spectra, containing 11 phenolic compounds, 12 phthalides and 1 amino acid. Three dicaffeoylquinic acids, 5-feruoylquinic acid and ferulic acid hexoside were detected in A. sinensis for the first time. According to Chinese medicine theories, the three parts of Danggui (Guitou, Guishen, Guiwei) have different therapeutic effects and their substantial evidence has not been clearly elucidated yet. In the present study, the chemical compositions of different parts of Danggui were investigated by using the established HPLC method. The result indicated that the content of tryptophan was getting lower and the contents of low polarity constituents like phthalides trended to get higher from Guitou to Guiwei. This might partly explain the therapeutic effects of different parts of Danggui.

CLC Number:

R284

Supporting:

Yanyan Wu, Lu Wang, Guangxue Liu, Feng Xu, Mingying Shang, Shaoqing Cai. Characterization of principal compositions in the roots of Angelica sinensis by HPLC-ESI-MSn and chemical comparison of its different parts[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(6): 393-402.

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