Development of a method for the quantitative determination of geniposide in rat plasma by ......

doi:10.5246/jcps.2013.03.033

Abstract

Abstract:

Geniposide is a major bioactive constituent isolated from Gardenia jasminoides Ellis. To evaluate the pharmacokinetics of geniposide in pre-clinical studies, a rapid and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated. After simple protein precipitation, geniposide was analyzed on a Diamonsil^® C18column with a mobile phase of 10 mM ammonium acetate and methanol (20:80, v/v) at a flow rate of 0.6 mL/min. Detection was performed in "Truncated" multiple-reaction monitoring (MRM) mode with positive electrospray ionization (ESI) at m/z 411→411 for geniposide, and MRM mode with negative ESI ionization at m/z 415→295 for puerarin (internal standard, IS). Linearity was established in the concentration range from 10.0 to 5000 ng/mL. The extraction recoveries ranged from 84.8% to 90.5% at concentrations of 10.0, 500 and 4.5×10³ ng/mL. The lower limit of quantification (LLOQ) was 10.0 ng/mL with 50 µL plasma. The validated method was successfully applied to the pharmacokinetic study of geniposide in rats at a dose of 200 mg/kg by oral administration.

Key words: LC-MS/MS, Protein precipitation, Rat plasma, Pharmacokinetics, Geniposide

CLC Number:

R917

Supporting:

Zhipeng Deng, Huixia Fan, Hao Zhong, Shuxiang Cui, Qingqiang Yao^*. Development of a method for the quantitative determination of geniposide in rat plasma by ......[J]. , DOI: 10.5246/jcps.2013.03.033.

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Development of a method for the quantitative determination of geniposide in rat plasma by ......

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