Simultaneous determination of steroidal saponins in Anemarrhena asphodeloides Bge. by ultra high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

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Simultaneous determination of steroidal saponins in Anemarrhena asphodeloides Bge. by ultra high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

Yang Zhao, Liping Kang, Heshui Yu, Xu Pang, Zhenfang Wang, Chengqi Xiong, Chao Liu, Lifeng Han, Xiumei Gao, Baiping Ma^*

1. Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing 100850, China
2. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China

Received:2013-03-04 Revised:2013-04-15 Online:2013-05-08 Published:2013-05-08
Contact: Baiping Ma*

Abstract

Abstract:

The objective of the research is to develop a quantitative method by ultra high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF MS) for the analysis of seven major steroidal saponins (timosaponin N, timosaponin E1, timosaponin BII, timosaponin B, anemarrhenasaponin I, anemarrhenasaponin A2, and timosaponin AIII) in Anemarrhena asphodeloides Bge. The complete separation of these seven steroidal saponins was achieved within 18 min with an ACQUITY UPLC HSS T3 column using an acetonitrile-water (contain 0.1% formic acid) gradient system. The limits of quantitation (LOQ), 0.18-0.75 ng/µL for seven steroidal saponins, were determined experimentally. The limits of detection (LOD) were found to be 0.05-0.22 ng/µL for these saponins. The correlation coefficients (r²) for calibration curves varied from 0.9902 to 0.9979. This method showed good repeatability for the quantification of these saponins in rhizomes of A. asphodeloides, with intra-day and inter-day variations of less than 5.0% for seven steroidal saponins. The recoveries of seven steroidal saponins were from 97.13% to 101.98%. The validated method was successfully applied to quantifying seven steroidal saponins in various sources of A. asphodeloides (different collecting places or processing methods) and Zhimu concentrate-granules (ZMCG).

Key words: Anemarrhena asphodeloides Bge., UHPLC/Q-TOF MS, Steroidal saponins, Simultaneous determination

CLC Number:

R284.1

Supporting:

Yang Zhao, Liping Kang, Heshui Yu, Xu Pang, Zhenfang Wang, Chengqi Xiong, Chao Liu, Lifeng Han, Xiumei Gao, Baiping Ma^*. Simultaneous determination of steroidal saponins in Anemarrhena asphodeloides Bge. by ultra high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry [J]. .

References

[1] Zhang, J.Y.; Zhang, M.Y.; Sugahara, K.; Yasuhiro, S.; Meng, Z.Y.; Xu, S.X.; Hiroyuki, K. Biochem. Biophys. Res. Commun. 1999, 259, 636-639.
[2] Kang, L.P.; Zang, J.; Cong, Y.; Li, B.; Xiong, C.Q.; Zhao, Y.; Tan, D.W.; Yu, H.S.; Yu, Z.Y.; Cong, Y.W.; Liu, C.; Ma, B.P. Planta Med. 2012, 78, 611-616.
[3] Deng, Y.; Ma, B.P.; Xu, Q.P.; Xiong, C.Q.; Liu, Z.Q.; Zhao, Y.; Zhang, A.L. Chin. Pharmacol. Bull. 2005, 21, 1460-1462.
[4] Cong, Y.; Liu, X.L.; Yu, Z.Y.; Kang, L.P.; Ma, B.P.; Cong, Y.W. Med. J. Chin. PLA. 2010, 35, 1370-1373.
[5] Kang, M.; Jung, I.; Hur, J.; Kim, S.H.; Lee, J.H.; Kang, J.Y.; Jung, K.C.; Kim, K.S.; Yoo, M.C.; Park, D.S. J. Ethnopharmacol. 2010, 131, 485-496.
[6] Deng, Y.; Ma, B.P.; Xu, Q.P.; Xiong, C.Q.; Liu, Z.Q.; Zhao, Y.; Chu, S.Y. Chin. Pharmacol. Bull. 2005, 21, 830-833.
[7] Li, T.J.; Qiu, Y.; Yang, P.Y.; Rui, Y.C.; Chen, W.S. Neurosci. Lett. 2007, 421, 147-151.
[8] Takeda, Y.; Togashi, H.; Matsuo, T. J. Gastroenterol. 2001, 36, 79-90.
[9] Li, C.M.; Gao, Y.L.; Li, M.; Han, B.; Liu, Z.F.; Pharmacol. Clin. Chin. Mater. Clin. Med. 2005, 21, 22-23.
[10] Ji, X.; Feng, Y.F. Chin. Tradit. Herb. Drugs. 2010, 41, Appendix 12-15.
[11] Yuan, Y.; Chen, W.S.; Sun, L.N.; Zheng, S.Q.; Lou, Z.Y.; Zhang, H.M. Chin. Tradit. Herb. Drugs. 2006, 37, 1574-1576.
[12] Wang, K.R.; Zhang, H.; Zhu, Z.Y.; Lou, Z.Y.; Zhao, L.; Chai, Y.F. Acad. J. Xi’an Jiaotong Univ. 2010, 22, 149-155.
[13] Kang, L.P.; Yu, K.; Zhao, Y.; Liu, Y.X.; Yu, H.S.; Pang, X.; Xiong, C.Q.; Tan, D.W.; Gao, Y.; Liu, C.; Ma, B.P. J. Pharm. Biomed. Anal. 2012, 62, 235-249.
[14] Zhu, J.; Guo, X.J.; Fu, S.P.; Zhang, X.L.; Liang, X.M. J. Pharm. Biomed. Anal. 2010, 53, 462-474.
[15] Pérez-Palacios, D.; Fernández-Recio, M.Á.; Moreta, C.; Tena, M.T. Talanta. 2012, 99, 167-174.
[16] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China. Vol 1, Beijing: China Medical Science and Technology Press. 2010, 197.
[17] Yang, D.Y.; Lu, T.J.; Hwang, L.S. Food Chem. 2009, 113, 155-159.