An HPLC method for the assay of trypsin inhibitors and its application to the study of Momordica cochinchinensis extract

doi:10.5246/jcps.2011.04.050

Abstract

Abstract: A reliable and validated HPLC method was established for the assay of trypsin inhibitors (TI) and it was used in the investigation of the active TI components in Momordica cochinchinensis (Cucurbitaceae family). The underlying principle of the assay is the measurement of the decrease in trypsin activity due to the presence of TI in analyzed samples, which was achieved by using HPLC separation and quantification of p-nitroanilide that was generated by tryptic hydrolysis of N-α-benzoyl-DL-arginine-4-nitroanilide. The results showed that the HPLC method had higher selectivity than conventional spectrophotometric assay.

Key words: HPLC, Momordica cochinchinensis, Trypsin inhibitor

CLC Number:

R917.01

Supporting:

Zhi-Yan Lin, Zhu-Hong Yu, Chen Chen, Qi-Xin Dong, Ming-Zhang Wang, Yun-Qiu Yu^* . An HPLC method for the assay of trypsin inhibitors and its application to the study of Momordica cochinchinensis extract [J]. , DOI: 10.5246/jcps.2011.04.050.

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