Metabolite identification and the development of a simultaneous quantification method for wogonin and wogonin-7-O-glucuronide: application to a distribution study in mice liver

doi:10.5246/jcps.2011.03.035

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Metabolite identification and the development of a simultaneous quantification method for wogonin and wogonin-7-O-glucuronide: application to a distribution study in mice liver

Wen-Yuan Liu, Xiao-Jing Yang, Feng Feng^*, Xiao-Zhen Xu

1. Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China
2. Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing 210009, China
3. Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009, China

Received:2011-01-04 Revised:2011-03-09 Online:2011-05-06 Published:2011-05-06
Contact: Feng Feng*

Abstract

Abstract:

Wogonin possesses potent inhibitory activities against cancer cell growth in vitro and in vivo and has attractive safety profiles. A highly sensitive liquid chromatography coupled with tandem mass spectrometry method was developed for the identification of major metabolites in mice liver after intravenous administration of wogonin. Five metabolites were identified and biotransformation pathways were elucidated as well. Furthermore, a method was developed and validated for the simultaneous quantitatively determination of wogonin and wogonin-7-O-glucuronide in mice liver. After liquid-liquid extraction by ethyl acetate, the analytes were separated on a C18 column with a mobile phase of methanol-10 mM ammonium acetate water (80:20, v/v). The detection was operated with negative selected reaction monitoring mode using electrospray ionization technique. The linear response range was 0.2-40 μg/g for both wogonin and wogonin-7-O-glucuronide in mice liver. The developed quantification method was suitable for distribution study after intravenous infusion of wogonin injection in animals.

Key words: Wogonin, Wogonin-7-O-glucuronide, Metabolite identification, Liquid chromatography-tandem mass spectrometry

CLC Number:

R969.1

Supporting:

Wen-Yuan Liu, Xiao-Jing Yang, Feng Feng^*, Xiao-Zhen Xu. Metabolite identification and the development of a simultaneous quantification method for wogonin and wogonin-7-O-glucuronide: application to a distribution study in mice liver[J]. , DOI: 10.5246/jcps.2011.03.035.

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Metabolite identification and the development of a simultaneous quantification method for wogonin and wogonin-7-O-glucuronide: application to a distribution study in mice liver

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