Comparative study on ginsenosides of ginsengs from different regions

doi:10.5246/jcps.2023.12.077

Abstract

Abstract:

Ginsenosides, unique bioactive components found in ginseng (Panax ginseng Meyer), can be influenced by the geographical area in which the ginseng is grown. In northeastern China, ginseng thrives in diverse living environments that play a significant role in shaping the composition of ginsenosides. This study aimed to explore the variations in ginseng constituents cultivated in different growing regions. UPLC coupled with triple quadrupole tandem time-of-flight mass spectrometry was employed to analyze and characterize the ginsenosides, while multivariate statistical analysis was utilized to identify differences among ginseng samples from various regions. A total of 359 ginsenosides, including 35 potential new compounds, were characterized in the different ginseng samples. Among them, 283 and 312 ginsenosides were identified in ginseng from non-traditional and traditional cultivation regions, respectively. These findings contributed to the expansion of the ginseng compound library and provided valuable insights for future research on ginseng.

Key words: Panax ginseng Meyer, Ginsenosides, Growing regions, Multivariate statistical analysis, UPLC-MS/MS

Supporting:

/Jwk3_zgyxen/attached/file/20240104/20240104222856_933.pdf

Yizheng Sun, Ying Qu, Xiaoyan Liu, Wei Xu, Youbo Zhang. Comparative study on ginsenosides of ginsengs from different regions[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(12): 961-970.

Figures/Tables 5

References 22

[1]	Xu, W.Q.; Choi, H.K.; Huang, L.F. State of panax ginseng research: a global analysis. Molecules. 2017, 22, 1518.
[2]	Wang, N.; Wang, X.L.; He, M.J.; Zheng, W.X.; Qi, D.M.; Zhang, Y.Q.; Han, C.C. Ginseng polysaccharides: a potential neuroprotective agent. J. Ginseng Res. 2021, 45, 211–217.
[3]	Li, H.L.; Huang, N.; Zhu, W.K.; Wu, J.C.; Yang, X.H.; Teng, W.J.; Tian, J.H.; Fang, Z.H.; Luo, Y.B.; Chen, M.; Li, Y. Modulation the crosstalk between tumor-associated macrophages and non-small cell lung cancer to inhibit tumor migration and invasion by ginsenoside Rh₂. BMC Cancer. 2018, 18, 1–12.
[4]	Gao, Y.; Li, J.T.; Wang, J.; Li, X.; Li, J.P.; Chu, S.F.; Li, L.; Chen, N.H.; Zhang, L. Ginsenoside Rg1 prevent and treat inflammatory diseases: a review. Int. Immunopharmacol. 2020, 87, 106805.
[5]	Tan, B.; Giangaspero, M.; Sun, N.; Jin, Y.P.; Liu, K.X.; Wang, Q.Y.; Cheng, S.P.; Wang, Y.P.; Zhang, S.Q. Antiviral effect of ginsenoside Rb2 and Rb3 against bovine viral diarrhea virus and classical swine fever virus in vitro. Front. Vet. Sci. 2021, 8, 764909.
[6]	Song, M.; Jia, F.B.; Cao, Z.; Zhang, H.Y.; Liu, M.L.; Gao, L. Ginsenoside Rg3 attenuates aluminum-induced osteoporosis through regulation of oxidative stress and bone metabolism in rats. Biol. Trace Elem. Res. 2020, 198, 557–566.
[7]	Qi, Z.; Chen, L.X.; Li, Z.; Shao, Z.J.; Qi, Y.L.; Gao, K.; Liu, S.X.; Sun, Y.S.; Li, P.Y.; Liu, J.P. Immunomodulatory effects of (24R)-pseudo-ginsenoside HQ and (24S)-pseudo-ginsenoside HQ on cyclophosphamide-induced immunosuppression and their anti-tumor effects study. Int. J. Mol. Sci. 2019, 20, 836.
[8]	Ru, W.W.; Wang, D.L.; Xu, Y.P.; He, X.X.; Sun, Y.E.; Qian, L.Y.; Zhou, X.S.; Qin, Y.F. Chemical constituents and bioactivities of Panax ginseng (C. A. mey.). Drug Discov. Ther. 2015, 9, 23–32.
[9]	Chunhao, Yu, . Adulteration and cultivation region identification of American ginseng using HPLC coupled with multivariate analysis. J. Pharm. Biomed. Anal. 2014, 99, 8–15.
[10]	Gao, Y.F.; Liu, Y.Y.; Yang, X.; Zhang, T.Y.; Hou, Y.; Wang, P.W.; Liu, Y.L.; Yuan, L.L.; Zhang, H.T.; Wu, C.F.; Yang, J.Y. Pseudoginsenoside-F11 ameliorates thromboembolic stroke injury in rats by reducing thromboinflammation. Neurochem. Int. 2021, 149, 105108.
[11]	Xing, Q.Q.; Liang, T.; Shen, G.B.; Wang, X.L.; Jin, Y.; Liang, X.M. Comprehensive HILIC × RPLC with mass spectrometry detection for the analysis of saponins in Panax notoginseng. Analyst. 2012, 137, 2239–2249.
[12]	Ma, C.; Guan, H.D.; Ju, Z.C.; Li, S.P.; Deng, G.; Zhang, Y.P.; Lin, Q.Y.; Cheng, X.M.; Yang, L.; Wang, Z.T.; Wang, C.H. Identification and characterization of forced degradation products and stability-indicating assay for notoginsenosidefc by using UHPLC-Q-TOF-MS and UHPLC-MS/MS: insights into stability profile and degradation pathways. J. Sep. Sci. 2019, 42, 1550–1563.
[13]	Wang, H.J.; Wu, R.M.; Xie, D.; Ding, L.Q.; Lv, X.; Bian, Y.Q.; Chen, X.; Nisma Lena, B.A.; Wang, S.C.; Li, K.; Chen, W.; Ye, G.; Sun, M.Y. A combined phytochemistry and network pharmacology approach to reveal the effective substances and mechanisms of Wei-fu-Chun Tablet in the treatment of precancerous lesions of gastric cancer. Front. Pharmacol. 2020, 11, 558471.
[14]	Xie, Y.Y.; Luo, D.; Cheng, Y.J.; Ma, J.F.; Wang, Y.M.; Liang, Q.L.; Luo, G.A. Steaming-induced chemical transformations and holistic quality assessment of red ginseng derived from Panax ginseng by means of HPLC-ESI-MS/MSn-based multicomponent quantification fingerprint. J. Agric. Food Chem. 2012, 60, 8213–8224.
[15]	Duan, S.N.; Qi, W.; Zhang, S.W.; Huang, K.K.; Yuan, D. Ultra high performance liquid chromatography coupled with electrospray ionization/quadrupole time-of-flight mass spectrometry for the rapid analysis of constituents in the traditional Chinese medicine formula Wu Ji Bai Feng Pill. J. Sep. Sci. 2017, 40, 3977–3986.
[16]	Wu, W.; Sun, L.; Zhang, Z.; Guo, Y.; Liu, S. Profiling and multivariate statistical analysis of Panax ginseng based on ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry. J. Pharm. Biomed. Anal. 2015, 107, 141–150.
[17]	Dai, D.; Zhang, C.F.; Williams, S.; Yuan, C.S.; Wang, C.Z. Ginseng on cancer: potential role in modulating inflammation-mediated angiogenesis. Am. J. Chin. Med. 2017, 45, 13–22.
[18]	Fan, W.X.; Huang, Y.L.; Zheng, H.; Li, S.Q.; Li, Z.H.; Yuan, L.; Cheng, X.; He, C.S.; Sun, J.F. Ginsenosides for the treatment of metabolic syndrome and cardiovascular diseases: Pharmacology and mechanisms. Biomed. Pharmacother. 2020, 132, 110915.
[19]	Lim, S.; Park, J.; Um, J.Y. Ginsenoside Rb1 induces beta 3 adrenergic receptor-dependent lipolysis and thermogenesis in 3T3-L1 adipocytes and db/db mice. Front. Pharmacol. 2019, 10, 1154.
[20]	Dai, S.; Hong, Y.; Xu, J.; Lin, Y.; Si, Q.; Gu, X. Ginsenoside Rb2 promotes glucose metabolism and attenuates fat accumulation via AKT-dependent mechanisms. Biomed. Pharmacother. 2018, 100, 93–100.
[21]	Choi, K.; Kim, M.; Ryu, J.; Choi, C. Ginsenosides compound K and Rh2 inhibit tumor necrosis factor-α-induced activation of the NF-κB and JNK pathways in human astroglial cells. Neurosci. Lett. 2007, 421, 37–41.
[22]	Li, J.F.; Shao, H.H.; Bi, Y.M.; Jiao, X.L.; Zhang, X.M.; Gao, W.W. Effects of nutrients deficiency on growth and saponin accumulation of American ginseng. Chin. J. Chin. Mater. Med. 2020, 45, 1866–1872.