Isolation of endophytic fungi with anti-inflammatory effect in vitro from Zanthoxylum armatum

doi:10.5246/jcps.2021.07.045

Journal of Chinese Pharmaceutical Sciences ›› 2021, Vol. 30 ›› Issue (7): 570-577.DOI: 10.5246/jcps.2021.07.045

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Isolation of endophytic fungi with anti-inflammatory effect in vitro from Zanthoxylum armatum

Aihong Zhao¹^,^#, Tianxia Wang¹^,^#, Xiaoyan Liu², Weihong Zhao¹, Jianping Ma¹, Xiuwei Yang², Tao Guo³^,^*(), Youbo Zhang²^,^*()

1 Key Laboratory of Herbal-Tebitan Drug Screening and Deep Processing of Gansu Province, Lanzhou University of Technology, Lanzhou 730050, China
2 State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
3 School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China

Received:2020-12-15 Revised:2021-02-10 Accepted:2021-02-16 Online:2021-07-27 Published:2021-07-27
Contact: Tao Guo, Youbo Zhang
About author:
# These authors contributed equally to this work.

Abstract

Abstract:

Endophytic fungus is a rich source of bioactive metabolites with multiple activities. In the present study, eight endophytic fungal strains (Z-1 to Z-8) from Zanthoxylum armatum DC. were isolated and identified, and the anti-inflammatory effect of their extracts was evaluated in vitro. The results showed that four endophytic fungal extracts (Fusarium oxysporum Schlecht. (Z-1), Alternaria arborescens E.G Simmons (Z-3), Mucor fragilis Bainier (Z-4) and Aspergillus terreus Thom (Z-6)) displayed good activities in inhibition of excessive production of nitric oxide in lipopolysaccharide-activated mouse macrophage RAW264.7 cells. Among them, the extracts of Fusarium oxysporum Schlecht showed the maximum inhibition activity with a half maximal inhibitory concentration of 174.0 μg/mL. The underlying mechanism of the anti-inflammatory effect of Fusarium oxysporum Schlecht extract was associated with its inhibition on the release of several inflammatory factors. The mRNA expression of interleukin 1β, interleukin 6, inducible nitric oxide synthase in lipopolysaccharide-activated mouse macrophage RAW264.7 cells were all inhibited by Z-1 extract detected by real-time polymerase chain reaction. In summary, several endophytic fungal from Zanthoxylum armatum have good anti-inflammatory activity and Fusarium oxysporum Schlecht possesses a promising potential to be developed to a novel anti-inflammatory agent.

Key words: Endophytic fungi, Zanthoxylum armatum, Anti-inflammation, Real-time polymerase chain reaction

Supporting:

Aihong Zhao, Tianxia Wang, Xiaoyan Liu, Weihong Zhao, Jianping Ma, Xiuwei Yang, Tao Guo, Youbo Zhang. Isolation of endophytic fungi with anti-inflammatory effect in vitro from Zanthoxylum armatum[J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(7): 570-577.

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References 16

[1]	Upreti, K.; Semwal, A.; Upadhyaya, K.; Masiwal, M. Pharmacognostical and phytochemical screening of leaf extract of Zanthoxylum armatum DC. Int. J. Herb. Med. 2013, 1, 6–11.
[2]	Samad, A.; Badshah, S.; Khan, D.; Ali, F.; Amanullah, M.; Hanrahan, J. New prenylated carbazole alkaloids from Zanthoxylum armatum. J. Asian Nat. Prod. Res. 2014, 16, 1126–1131.
[3]	Guo, T.; Li, Y.H. Determination of four lignanoids in roots, stems and leaves of Zanthoxylum armatum DC by HPLC-DAD with HPLC-ESI-QTOF-MS confirmation. J. Anal. Chem. 2016, 71, 527–533.
[4]	Guo, T.; Xie, H.; Deng, Y.X.; Pan, S.L. A new lignan and other constituents from Zanthoxylum armatum DC. Nat. Prod. Res. 2012, 26, 859–864.
[5]	Ma, L.; Xi, J.Q.; Cao, Y.H.; Wang, X.Y.; Zheng, S.C.; Yang, C.G.; Yang, L.L.; Mi, Q.L.; Li, X.M.; Zhu, M.L.; Mo, M.H. Bacillus endozanthoxylicus sp. nov., an endophytic bacterium isolated from Zanthoxylum bungeanum Maxim leaves. Int. J. Syst. Evol. Microbiol. 2017, 67, 3699–3705.
[6]	Ferchichi, N.; Toukabri, W.; Boularess, M.; Smaoui, A.; Mhamdi, R.; Trabelsi, D. Isolation, identification and plant growth promotion ability of endophytic bacteria associated with lupine root nodule grown in Tunisian soil. Arch. Microbiol. 2019, 201, 1333–1349.
[7]	Li, P.Q.; Wu, Z.; Liu, T.; Wang, Y.N. Biodiversity, phylogeny, and antifungal functions of endophytic fungi associated with Zanthoxylum bungeanum. Int. J. Mol. Sci. 2016, 17, 1541.
[8]	Gilani, S.N.; Khan, A.U.; Gilani, A.H. Pharmacological basis for the medicinal use of Zanthoxylum armatum in gut, airways and cardiovascular disorders. Phytother. Res. 2010, 24, 553–558.
[9]	Moharram, A.M.; Zohri, A.A.; Omar, H.M.; El-Ghani, O.A.A. In vitro assessment of antimicrobial and anti-inflammatory potential of endophytic fungal metabolites extracts. Eur. J. Biol. Res. 2017, 7, 234–244.
[10]	Mondol, M.A.M.; Farthouse, J.; Islam, M.T.; Schüffler, A.; Laatsch, H. Metabolites from the endophytic fungus curvularia sp. M12 act as motility inhibitors against phytophthora capsici zoospores. J. Nat. Prod. 2017, 80, 347–355.
[11]	Choi, E.M.; Hwang, J.K. Screening of Indonesian medicinal plants for inhibitor activity on nitric oxide production of RAW264.7 cells and antioxidant activity. Fitoterapia. 2005, 76, 194–203.
[12]	Singh, T.D.; Meitei, H.T.; Sharma, A.L.; Robinson, A.; Singh, L.S.; Singh, T.R. Anticancer properties and enhancement of therapeutic potential of cisplatin by leaf extract of Zanthoxylum armatum DC. Biol. Res. 2015, 48, 46.
[13]	Zheng, C.J.; Xu, L.L.; Li, Y.Y.; Han, T.; Zhang, Q.Y.; Ming, Q.L.; Rahman, K.; Qin, L.P. Cytotoxic metabolites from the cultures of endophytic fungi from Panax ginseng. Appl. Microbiol. Biotechnol. 2013, 97, 7617–7625.
[14]	Liu, X.L.; Huang, K.H.; Zhou, J.Z.; Meng, L.; Wang, Y.; Zhang, L.X. Identification and antibacterial characteristics of an endophytic fungus Fusarium oxysporum from Lilium lancifolium. Lett. Appl. Microbiol. 2012, 55, 399–406.
[15]	Muscará, M.N.; de Nucci, G. Simultaneous determination of nitrite and nitrate anions in plasma, urine and cell culture supernatants by high-performance liquid chromatography with post-column reactions. J. Chromatogr. B Biomed. Sci. Appl. 1996, 686, 157–164.
[16]	Zhang, Y.B.; Yan, T.T.; Sun, D.X.; Xie, C.; Zheng, Y.R.; Zhang, L.; Yagai, T.; Krausz, K.W.; Bisson, W.H.; Yang, X.W.; Gonzalez, F.J. Structure-activity relationships of the main bioactive constituents of Euodia rutaecarpa on aryl hydrocarbon receptor activation and associated bile acid homeostasis. Drug Metab. Dispos. Biol. Fate Chem. 2018, 46, 1030–1040.

Isolation of endophytic fungi with anti-inflammatory effect in vitro from Zanthoxylum armatum

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