Preparation of a Hippophae rhamnoides Linn oil nanoemulsion and the visualization of its transdermal permeation

doi:10.5246/jcps.2019.08.056

Abstract

Abstract:

A Hippophae rhamnoides Linn oil nanoemulsion was developed, and its physicochemical properties and in vitro transdermalcharacteristics were investigated. Then its skin irritation was investigated.The optimum Hippophae rhamnoides Linn oil nanoemulsion prescription was determined using Cremophor EL as an emulsifier, Transcutol P as a co-emulsifier, and isopropyl myristate (IPM) as the oil phase (IPM%:Cremophor EL%:Hippophae rhamnoides Linn oil% = 5:16:20, Km = 4:1). The prepared Hippophaerhamnoides Linn oil nanoemulsion was pale yellow and transparent. Globular droplets were observed under a transmission electronmicroscope (TEM). The average particle size was 52.2±4.8 nm. The loading capacity was 10.68%, and the oil-in-water (O/W) type nanoemulsion was stable after centrifugation. The CLSM results showed that the fluorescence intensity of the OB Hippophae rhamnoides Linn oil nanoemulsion group was stronger than that of the Hippophae rhamnoides Linn oil cream and Hippophae rhamnoides Linn oil groups in each layer of skin. The study also showed that skin surface treated with the Hippophae rhamnoides Linn oil nanoemulsion had strong fluorescence. The skin depth had weak fluorescence, while the areas near the hair follicle and its appendages had the strongest fluorescence. The skin irritation test showed that the prepared Hippophae rhamnoides Linn oil nanoemulsion was applied to the normal and damaged skin of the rabbit, and there was no apparent redness and swelling. These results suggested that the nanoemulsion coated with Hippophae rhamnoides Linn oil was a potential delivery system for skin disorders.

Key words: Hippophae rhamnoides Linn oil, Nanoemulsion, Confocal laser scanning microscopy, Phase diagram

CLC Number:

R943

Supporting:

Qi Shen, Mei Wang, Xiaohui Tang, Munire Kuerban. Preparation of a Hippophae rhamnoides Linn oil nanoemulsion and the visualization of its transdermal permeation[J]. Journal of Chinese Pharmaceutical Sciences, 2019, 28(8): 579-594.

References

[1] Ji, B.Y.; Liu, X.H.; Sun, C. To prospect the application of Hippophae rhamnoides Linn in the field of Medicine. Hippophae. 2005, 8, 36–37.

[2] Wei, Z.Y.; Chen, J.; Zhang, H. Active constituents of Hippophae rhamnoides Linn and their medical applications. Journal of Xinzhou Teacher University. 2010, 26, 46–48.

[3] Upadhyay, N.K.; Kumar, R.; Mandotra, S.K.; Meena, R.N.; Siddiqui, M.S.; Sawhney, R.C.; Gupta, A. Safety and healing efficacy of Sea buckthorn (Hippophae rhamnoides L.) seed oil on burn wounds in rats. Food Chem. Toxicol. 2009, 47, 1146–1153.

[4] Che, X. P.; Huo, H.R. The analgesic effect of Hippophae rhamnoides Linn oil on mice and its effect on experimental gastric ulcer in rats. Hippophae. 2001, 1, 34–37.

[5] Che, X.P.; Huo, H.R. Anti-inflammatory effect and toxicity test of Hippophae rhamnoides Linn oil suppository topical administration to mice. Hippophae. 1995, 3, 40–44.

[6] Zhang, W.L.; Zhang, Z.H.; Fan, J.J. Experimental observation of the treatment of acute radiation dermatitis and the preliminary results of clinical application. Hippophae. 1988, 1, 27–29.

[7] Yang, B.; Kalimo, K.O.; Ma, L.M.; Kallio, S.E.; Katajisto, J.K.; Peltola, O.J.; Kallio, H.P. Effects of dietary supplementation with sea buckthorn (Hippophaë rhamnoides) seed and pulp oils on atopic dermatitis. J. Nutr. Biochem. 1999, 10, 622–630.

[8] Wu, F.H. A series of drug studies of the Soviet Union about the Hippophae rhamnoides Linn. Hippophae. 1991, 2, 38–41.

[9] Zhao, Y.S. The clinical report of 32 cases of scalding and scalding burned by Hippophae rhamnoides Linn seed oil was reported. Hippophae. 1994, 3, 36–37.

[10] Zhang, X.T; Fan, Y.L.; Kang, A.J. Study on the effects of Hippophae rhamnoides Linn oil on the effect of the experimental tympanal perforation healing process. Journal of shanxi medical science. 2000, 4, 253–255.

[11] Amjt, K.; Varshney, A.C.; Tyagi, S.P. Efficacy of Hippophae rhamnoides Linn (Hippophae Rhanoides) Oil in the Healing of Cutaneous Burns in Bovines. The Indian Veterinary Journal. 2012, 189, No.2.

[12] Rezaee, M.; Basri, M.; Rahman, R.N.; Salleh, A.B.; Chaibakhsh, N.; Karjiban, R.A. Formulation development and optimization of palm kernel oil esters-based nanoemulsions containing sodium diclofenac. Int. J. Nanomedicine. 2014, 9, 539–548.

[13] Pey, C.M.; Maestro, A.; Solé, I.; González, C.; Solans, C.; Gutiérrez, J.M. Optimization of nano-emulsions prepared by low-energy emulsification methods at constant temperature using a factorial design study. Colloids Surf A Physicochem. Eng. Aspects. 2006, 288, 144–150.

[14] Kreilgaard, M.; Pedersen, E.J.; Jaroszewski, J.W. NMR characterisation and transdermal drug delivery potential of microemulsion systems. J. Control. Release. 2000, 69, 421–433.

[15] Lin, Y.H.; Tsai, M.J.; Fang, Y.P.; Fu, Y.S.; Huang, Y.B.; Wu, P.C. Microemulsion formulation design and evaluation for hydrophobic compound: Catechin topical application. Colloids Surf. B Biointerfaces. 2018, 161, 121–128.

[16] Zhang, L.C.; Hu, J.H. The research progress of microemulsion system for microemulsion. Overseas medical pharmacy branch. 2004, 31, 44.

[17] Liu, H.Z.; Li, S.M.; Wang, Y.J.; Han, F.; Dong, Y. Bicontinuous water-AOT/Tween85-isopropyl myristate microemulsion: a new vehicle for transdermal delivery of cyclosporin A. Drug Dev. Ind. Pharm. 2006, 32, 549–557.

[18] Kemken, J.; Ziegler, A.; Müller, B.W. Influence of supersaturation on the pharmacodynamic effect of bupranolol after dermal administration using microemulsions as vehicle. Pharm. Res. 1992, 9, 554–558.

[19] Seo D.H.; Han H.D.; Chi S.C.; Shin B.C. Nathalia Rodríguez-Burneo|Maria Antònia Busquets|Joan Estelrich Preparation of phospholipid nanoemulsions loaded with paclitaxel. J. Korean Pharm. Sci. 2004, 34, 125–130.

[20] He J.; Yao X.L.; Feng G.R.; Wang R.; Yang B.L. Preparations of the Nanoemulsion Grapefruit Essential Oil and its quality evaluation. Food Res. Development. 2015, 36, 3–6.

[21] Peltola, S.; Saarinen-Savolainen, P.; Kiesvaara, J.; Suhonen, T.M.; Urtti, A. Microemulsions for topical delivery of estradiol. Int. J. Pharm. 2003, 254, 99–107.

[22] Mouri, A.; Legrand, P.; El Ghzaoui, A.; Dorandeu, C.; Maurel, J.C.; Devoisselle, J.M. Formulation, physicochemical characterization and stability study of lithium-loaded microemulsion system. Int. J. Pharm. 2016, 502, 117–124.

[23] de Mattos, C.B.; Argenta, D.F.; Melchiades, Gde L.; Cordeiro, M.N.; Tonini, M.L.; Moraes, M.H.; Weber, T.B.; Roman, S.S.; Nunes, R.J.; Teixeira, H.F.; Steindel, M.; Koester, L.S. Nanoemulsions containing a synthetic chalcone as an alternative for treating cutaneous leshmaniasis: optimization using a full factorial design. Int. J. Nanomedicine. 2015, 10, 5529–5542.

[24] Cavalcanti, A.L.M.; Reis, M.Y.F.A.; Silva, G.C.L.; Ramalho, Í.M.M.; Guimarães, G.P.; Silva, J.A.; Saraiva, K.L.A.; Damasceno, B.P.G.L. Microemulsion for topical application of pentoxifylline: In vitro release and in vivo evaluation. Int. J. Pharm. 2016, 506, 351–360.

[25] Li, W.H.; Wang, Y.Z.; Luo, S.X.; Zhang, Y.; Nie, R.J.; Sun, X.Y.; Li, F.Y.; Zhang, C.N. Study on transdermal mechanism of Sophora flavescens alkaloids nanoemulsions and nanoemulsion-based gels. Chin. Tradit. Herb. Drugs. 2017, 48, 484–489.

[26] Gurvinder, K. Development and optimization of nanoemulsion gel for topical delivery of imiquimod. J. Chin. Pharm. Sci. 2018, 27, 31–39.

[27] Goldberg-Cettina, M.; Liu, P.C.; Nightingale, J.; Kurihara-Bergstrom, T. Enhanced transdermal delivery of estradiol in vitro using binary vehicles of isopropyl myristate and short-chain alkanols. Int. J. Pharm. 1995, 114, 237–245.

[28] Zhu, X.L.; Chen, Z.L.; Li, G.F.; Zeng, K. Visualization and Quantification Research of Skin Permeation of Nanoemulsion. China Pharmacy. 2007, 18, 757–759.