Isolation, characterization and antioxidant activities of the endophytic fungi from Hosta. Ventricosa

doi:10.5246/jcps.2018.10.073

Journal of Chinese Pharmaceutical Sciences ›› 2018, Vol. 27 ›› Issue (10): 711-718.DOI: 10.5246/jcps.2018.10.073

• Original articles • Previous Articles Next Articles

Isolation, characterization and antioxidant activities of the endophytic fungi from Hosta. Ventricosa

Nan Chen, Xiaoxia Liang^*

Natural Medicine Research Center, College of veterinary medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2018-05-31 Revised:2018-06-19 Online:2018-10-30 Published:2018-07-06
Contact: Tel.: +86-028-86291470, E-mail: liangxiaoxia@sicau.edu.cn
Supported by:
National Natural Science Foundation of China (Grant No. 81703387) and the Foundation for the demonstration of tea-fruit-medicine organic ecological safety belt in Ya’an.

Abstract

Abstract:

A total of 13 types of endophytic fungi, classified into six genera, were isolated from Hosta. ventricosa based on 18S rDNA sequencing of ITS region and microscopic examination. The antioxidant activities of the crude extracts were investigated by T-AOC, DPPH and ABTS⁺ methods. The results showed that the crude extracts from Fusarium oxysporum (HoV1) and Epicoccum (HoV4), which presented higher phenol levels (1.90±0.03 mg/g, and 2.41±0.01 mg/g, respectively), exhibited stronger comprehensive antioxidant activities, with higher T-AOC values (63.46±0.26 U/g, and 66.05±0.71 U/g, respectively), lower EC₅₀ values against ABTS⁺(30.25±0.05 μg/mL, and 24.66±0.65 μg/mL, respectively)and DPPH radicals (83.42±0.24 μg/mL, and 59.136 μg/mL, respectively). Moreover, Fusarium oxysporum (HoV8) showed excellent scavenging activity against DPPH radicals (EC₅₀= 16.02 μg/mL), which was better than BHT. All of them could be useful in the developing of new natural antioxidant agents.

Key words: Hosta. Ventricosa, Endophytic fungi, Antioxidant activities, Total phenol contents

CLC Number:

R284

Supporting:

Nan Chen, Xiaoxia Liang. Isolation, characterization and antioxidant activities of the endophytic fungi from Hosta. Ventricosa[J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(10): 711-718.

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Isolation, characterization and antioxidant activities of the endophytic fungi from Hosta. Ventricosa

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