Simultaneous determination of Saikosaponin a, c and d in different parts of wild bupleurum from Shanxi Province

doi:10.5246/jcps.2018.10.072

Abstract

Abstract:

Asa traditional Chinese herbal medicine exhibiting analgesic, fever-reducing and anti-inflammatory effects, Radix Bupleuri (Chai-Hu) is commonly used for the treatment of influenza, which is derived from the dried roots of Bupleurum chinense DC. and Bupleurum scorzonerifolium Willd. Among of diverse chemical components, saikosaponins are the key active components of the herb medicine. In the present study, we established a method of high performance liquid chromatography (HPLC) coupled with evaporative light scattering detection (ELSD) for simultaneous determination of saikosaponin a, c and d in root, stem, leaf and flower of Bupleurumchinense (B chinense) collected from different areas of Shanxi Province, China. The results from 16 samples of root, stem, leaf and flower of B chinense demonstrated that the total contents of the three saikosaponins in the root of B chinense collected from Dongshan Taiyuan, Xishan, Tianlongshan and Pangquangou were 4.26 mg/g, 3.22 mg/g, 4.23 mg/g and 3.05 mg/g, respectively. However, there was scarcely any saikosaponins in the stem, leaf and flower of B chinensecollected from above-mentioned areas. The method of HPLC coupled with ELSD was suitable for quality control of Radix Bupleuri. The result also confirmed that the root of B chinense was the best medicinal part.

Key words: Bupleurum chinense DC., Different parts, Saikosaponins, Content analysis

CLC Number:

R284

Supporting:

Rui Wang, Ximei Yuan, Boya Ju, Rui Liu, Hongxia Yuan. Simultaneous determination of Saikosaponin a, c and d in different parts of wild bupleurum from Shanxi Province[J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(10): 703-710.

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