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Journal of Chinese Pharmaceutical Sciences ›› 2015, Vol. 24 ›› Issue (3): 177-184.DOI: 10.5246/jcps.2015.03.022

• Original articles • Previous Articles     Next Articles

An improved calibrated normalized resolution product (r*') and the application in HPLC fingerprints of soy isoflavone extract

Weiyang Shen1,2*, Juan Bian1,2, Feiye Liu1,2, Shuifang Peng1,2   

  1. 1. Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China
    2. Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China
  • Received:2014-10-01 Revised:2014-12-18 Online:2015-03-29 Published:2014-12-29
  • Contact: Tel.:18061297399
  • Supported by:
    National Higher-Education Institution General Research and Development Funding (Grant No. JKP2011010).

Abstract:

In this paper, we proposed a novel resolution criterion (improved calibrated normalized resolution product, r*') to evaluate separation quality of fingerprints. By comparing with the calibrated normalized resolution product (r*) and the hierarchical chromatographic response function (HCRF), the validity of this criterion was demonstrated by experimental chromatograms. The soy isoflavone extract was selected as the analytical object. The initial and end percentages of methanol and elution time affectinggradient elution were tested by orthogonal design. The final optimized conditions were as follows. It was detected by UV absorbance at 254 nm, column temperature was maintained at 36 ºC, solvent A was 0.1% (v/v) acetic acid, solvent B was methanol, gradient elution was from 34% to 65% B in a linear gradient in 25 min, and the flow-rate was set at 1.0 mL/min. In addition, the main ingredients of the soy isoflavone extract were confirmed by LC-ESI/MS.

Key words: Improved calibrated normalized resolution product, Fingerprints, Soy isoflavones, LC-ESI/MS

CLC Number: 

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