Folate and cell penetrating peptide modification effects on penetration behavior of nanostructured lipid carriers using multicellular tumor spheroids

doi:10.5246/jcps.2015.10.085

Journal of Chinese Pharmaceutical Sciences ›› 2015, Vol. 24 ›› Issue (10): 666-672.DOI: 10.5246/jcps.2015.10.085

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Folate and cell penetrating peptide modification effects on penetration behavior of nanostructured lipid carriers using multicellular tumor spheroids

Hongmeng Zhuang^1,2, Tingting Meng^1,2, Wei Gao², Lan Yuan³, Xianrong Qi^1,2*

1. Beijing Key Laboratory of Molecular Pharmaceutics and New drug delivery System, Peking University Health Science Center, Beijing 100191, China
2. Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
3. Medical and Healthy Analytical Center, Peking University Health Science Center, Beijing 100191, China

Received:2012-06-13 Revised:2012-07-25 Online:2015-10-21 Published:2012-08-13
Contact: Tel.: 86-10-82801708, E-mail: qixr@bjmu.edu.cn
Supported by:
National key Basic Research Program (Grant No. 2013CB932501), National Natural Science Foundation of China (Grant No. 81273454 and 81473156), Beijing National Science Foundation (Grant No. 7132113), and Doctoral Foundation of the Ministry of Education (Grant No. 20130001110055).

Abstract

Abstract:

Surface modification may have important influences on the penetration behavior of nanoscale drug delivery system. In the present study, we mainly focused on whether cell targeting or cell penetration could affect penetration abilities of nanostructuredlipid carriers (NLC). Real-time penetration of folate- or cell penetrating peptide (CPP)-modified NLC was evaluated using a multicellular tumor spheroid (MTS) established by stacking culture method as an in vitro testing platform. The results suggested that CPP modification had a better penetration behavior both on penetration depth and intensity compared with folate-modified NLC at the early stage of penetration process.

Key words: Nanostructured lipid carriers, Multicellular tumor spheroids, Stacking method, Folate, Cell penetrating peptide

CLC Number:

R945

Supporting:

Hongmeng Zhuang, Tingting Meng, Wei Gao, Lan Yuan, Xianrong Qi. Folate and cell penetrating peptide modification effects on penetration behavior of nanostructured lipid carriers using multicellular tumor spheroids[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(10): 666-672.

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Folate and cell penetrating peptide modification effects on penetration behavior of nanostructured lipid carriers using multicellular tumor spheroids

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