Pharmacokinetic and tissue distribution studies of paeoniflorin and albiflorin in rats after oral administration of total glycosides of paeony by HPLC-MS/MS

doi:10.5246/jcps.2014.06.054

Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (6): 403-411.DOI: 10.5246/jcps.2014.06.054

• Original articles • Previous Articles Next Articles

Pharmacokinetic and tissue distribution studies of paeoniflorin and albiflorin in rats after oral administration of total glycosides of paeony by HPLC-MS/MS

Minmin Zhao, Meng Gao, Yulu Tian, Yingfeng Du, Chunying Wang, Huijun Xu, Lantong Zhang, Qiao Wang^*

Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China

Received:2014-04-23 Revised:2014-05-04 Online:2014-06-28 Published:2014-05-05
Contact: Tel.: 86-311-86266419, Fax: 86-311-86052053
Supported by:
Natural Science Foundation of Hebei Province of China (Grant No. C2011206158, 08B031) and Hundreds of Innovative Talents Project of Hebei Education Department of China.

Abstract

Abstract:

A simple, sensitive and specific high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method combined with solid phase extraction has been developed and validated for the simultaneous quantification of paeoniflorin and albiflorin in rats plasma and tissue homogenate after administration of total glycosides of paeony (TGP). Chromatographic separation was achieved on a C₁₈column (150 mm×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile–0.05% formic acid aqueous (20: 80, v/v) at a flow rate of 0.5 mL/min. The analytes were detected with triple-quadrupole mass spectrometer using turbo ion spray with negative ionization in the multiple reaction-monitoring (MRM) modes. The results of method validationconformed to the requirements for the determination of biological samples. This method was successfully applied to the pharmacokinetics and tissue distribution study of TGP in rats. The results showed that paeoniflorin and albiflorin were absorbed and reached peak value quickly, and had the similar pharmacokinetic characteristics. Both of them underwent a rapid and wide distribution in the tissues throughout the whole body, among which stomach was the main distribution tissue. Moreover, they had the ability to cross the blood-brain barrier.

Key words: Total glycosides of paeony, Solid-phase extraction, Albiflorin, Paeoniflorin, HPLC-MS/MS

CLC Number:

R969

Supporting:

Minmin Zhao, Meng Gao, Yulu Tian, Yingfeng Du, Chunying Wang, Huijun Xu, Lantong Zhang, Qiao Wang. Pharmacokinetic and tissue distribution studies of paeoniflorin and albiflorin in rats after oral administration of total glycosides of paeony by HPLC-MS/MS[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(6): 403-411.

References

[1] Song, L.R. Modern Chinese Materia Medica Dictionary, Beijing, People’s Medical Publishing, 2001, 677-680.

[2] Wang, Q.; Liu, R.X.; Bi, K.S.; Guo, D.A. Chin. Tradit. Herb. Drugs. 2005,36, 1630-1632.

[3] Zhou, X.G.; Chen, S.X.; Wei, D.H.; Sun, S.Q.; Zong, M. Med. Plant. 2013, 4, 70-73.

[4] Ou, J.M.; Wu, D.L.; Jin, C.S.; Huang, Q.; Zhang, W. J. Chin. Med. Mater. 2013, 36, 423-425.

[5] Jin, C.S.; Cai, Y.J.; Wu, D.L. J. Chin. Med. Mater. 2010, 33, 1548-1550.

[6] Ji, J. China Med. Pharm. 2012, 2, 63-64.

[7] Li, H.; Wen, L. Chin. Hosp. Pharm. J. 2011, 31, 283-286.

[8] Zhang, C.Q.; Wu, X.X.; Wu, Y.G.; Xu, X.X.; Zhang, W.; Wang, K. Chin. Pharmacol. Bull. 2014, 30, 354-359.

[9] Zheng, L.Y.; Pan, J.Q.Guangzhou Med. J. 2011, 42, 66-69.

[10] Kang, J.; Kan, Q.C.; Zhang, X.J.; Du, S.Z. Chin. Hosp. Pharm. J. 2013, 33, 1542-1544.

[11] Li, B.F.; Wang, J.; Zhang, B.L. Drugs & Clinic. 2013, 28, 811-814.

[12] Liu, D.L.; Ma, S.T. Chin. J. Integr. Tradit. West. Med. 2013, 33, 1143-1146.

[13] Feng, W.L.; Wu, H.T.; Luo, C.H. Lishizhen Med. Mater. Med. Res. 2012, 23, 1778-1779.

[14] Hu, Z.Y.; Xu, L.; Yan, R.; Huang, Y.; Liu, G.; Zhou, W.X.; Zhang, Y.X. China J. Chin. Mater. Med. 2013, 38, 297-301.

[15] Zhang, Y.C.; Sun, H.S.; Pan, Z.L.; Li, M. World Clin. Drugs. 2010, 31, 449-453.

[16] Feng, L.; Ma, L. West China Med. J. 2013, 28, 1423-1425.

[17] Pan, H.M.; Cao, W.L.; Wang, X.G.; Niu, L.Y. Pharmacol. Clin. Chin. Mater. Med. 2013, 29, 59-61.

[18] Liu, E.L.; Xie, M.; Wang, Y.F.; Li, X.J.; Niu, L.Y. Chin. Pharm. J. 2012, 47, 534-538.

[19] Zhang, L.F.; Liu, M.Y.; Pan, H.M.; Shi, Z.; Wang, X.G.; Niu, L.Y. Chin. Pharmacol. Bull. 2011, 27, 1462-1466.

[20] Han, L.; Zhang, L.F.; Zhang, X.L.; Wang, X.G.; Niu, L.Y. Pharmacol. Clin. Chin. Mater. Med. 2012, 28, 69-71.

[21] Zhang, L.F.; Li, X.J.; Wang, Y.F.; Liu, M.Y.; Wang, X.G.; Niu, L.Y. Nat. Prod. Res. Dev. 2012, 24, 1040-1046.

[22] Liu, B.; Yang, Z.Y.; Wei, W. Acta Univ. Med. Anhui. 2009, 44, 707-710.

[23] Wu, J.; Yao, N.; Wang, D.W. China J. Chin. Mater. Med. 2008, 33, 2369-2372.

[24] Yang, X.G.; Zhang, L.B.; Pan, W.S.; Zhang, J.Y. Chin. Pharm. J. 2006, 41, 854-857.

[25] Ding, G.C.; Wang, F.F.; Ye, X.L. Northwest Pharm. J. 2009, 24, 124-125.

Pharmacokinetic and tissue distribution studies of paeoniflorin and albiflorin in rats after oral administration of total glycosides of paeony by HPLC-MS/MS

RichHTML

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 9

Recommended Articles

Metrics

Comments

[1]	Sisi Cao, Yang Deng, Hualin Cai, Zhenyan Hou, Miao Yan, Bikui Zhang. Pharmacokinetics and bioequivalence analysis of amlodipine tablets in Chinese female and male volunteers by HPLC-MS/MS [J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(4): 291-297.
[2]	Gongwen Liang, Na Li, Liping Ma, Libo Zhao, Luwen Shi. HPLC-MS/MS quantification of the HIV-1 protease inhibitor saquinavir in mice plasma and brain [J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(2): 106-114.
[3]	Xinhua Fang, Xin Wu, Xuemei Zhu, Hao Cai, Gang Cao. Albiflorin attenuates inflammatory injury by regulating the TLR4 signaling pathway and its negative regulating factor Tollip in experimental models of ulcerative colitis [J]. Journal of Chinese Pharmaceutical Sciences, 2016, 25(5): 366-372.
[4]	Hui-Lian Huang, Ke-Lan Liu, Feng Shao, Gang Ren, Yao-Hui Ye, Shou-Wen Zhang, Rong-Hua Liu^*. Rapid determination of quercetin-3'-O-glucoside in rat plasma by high performance liquid chromatography-tandem mass spectrometry [J]. , 2012, 21(3): 219-225.
[5]	Xu Zhu, Ya-Xin Sun, Xiao-Jing He, Feng Qiu, Jesse Ling Li, Li-Mei Zhao^* . HPLC-UV method with solid-phase extraction for the quantitative determination of biapenem in human plasma and its application in pharmacokinetic study [J]. , 2011, 20(1): 70-76.
[6]	Wen-Qian Chen, Yu-Hui Hu, Yan-Qing Zhang, Guan-Min Zhang, Liang Li, Wei-Ning Yang, Wei Lu^*. Population pharmacokinetics of paeoniflorin in guanxin II prescription [J]. , 2008, 17(1): 55-63.
[7]	MA Lei, YIN Li-li, SUN Jin, HE Zhong-gui^*. A Sensitive High-Performance Liquid Chromatography Coupled with Solid- Phase Extraction for Determination of Fexofenadine in Beagle Plasma [J]. , 2005, 14(4): 246-249.
[8]	Ding Jinsong, Zhang Bikui, Li Huande, Liu Yizhao, Deng Hang . HPLC Determination of Captopril in Human Plasma with Pre-column Derivation and Solid-phase Extraction and Studies on Its Pharmacokinetic and Relative Bioavailability [J]. , 2001, 10(3): 152-156.
[9]	Yi-Sheng Yuan, Min Shen. Study on Pharmacokinetics and Bioavailability of Terbutalinc by High-performance Liquid Chromatography with Electrochemical Detection [J]. , 1994, 3(2): 152-156.