Abstract: The sponge-associated fungus Nectria sp. HLS206, which belongs to the order of Hypocreales, was isolated from the marine sponge Gelliodes carnosa collected from the South China Sea. The secondary metabolites of the fungus were isolated. From the solid cultures, five compounds were purified, which are chalmicrin (1), hypocrealesate (2), fucosterol (3), stigmasterol (4) and β-sitosterol (5). The structures of the 5 compounds were elucidated by ESI-MS, 1D and 2D NMR spectra (COSY, HMQC and HMBC). To the best of our knowledge, it is the first time compound 1 was isolated from the order of Hypocreales, and the spectral data of compound 2 are also firstly reported.

Key words: Sponge-associated fungus, Nectria sp., Secondary metabolites, Spectral data