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Comparative analysis of essential oil components in Ephedra and its processed products by GC-MS

Ling-Yun Zhong*, Qian-Feng Gong, Jing Zhu, Fang Chen   

  1. Pharmaceutical College, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
  • Received:2009-09-29 Revised:2009-11-15 Online:2010-01-15 Published:2010-01-15
  • Contact: Ling-Yun Zhong*

Abstract: The similarities and differences of essential oil components in the raw ingredients of Ephedra (RIE) and processed products of Ephedra obtained by stir-baking with honey or stir-baking without any supplements were analyzed by GC-MS. Wet distillation (WD) and supercritical fluid extraction (SFE) were used to extract essential oil components from the samples. Total 48, 57 and 48 compounds were found in the extracted essential oils using WD from RIE, the products obtained by stir-baking with honey from Ephedra (SBHE) and the products obtained by stir-baking without any supplements from Ephedra (SBE), respectively; whereas total 22, 36 and 28 compounds were identified in the extracted essential oils using SPE from these three samples, respectively. In addition, 14 and 9 new compounds were found in the essential oils extracted using WD from SBHE and SBE, whereas 15 and 23 new compounds were found in the essential oils extracted by SFE from SBHE and SBE, respectively. The composition and concentration of the essential oil components in the processed products were significantly different from RIE. Such changes in essential oil components might affect drug actions, which is dependent on the manner in which the sample is processed. The findings in this study may shed some lights on the understanding and further exploration of Ephedra processing.

Key words: Ephedra essential oil, Ephedra processing, GC-MS

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