Simultaneous determination of four nucleosides in Carthamus tinctorius L. and Safflower injection using high-performance liquid chromatography

Abstract

Abstract: We quantitatively determined four nucleosides, including cytidine, uridine, guanosine, and adenosine, in Carthamus tinctorius L. and Safflower injection. Separation was performed on a Zorbax Eclipse XDB-18 column using a gradient elution with mobile phases of 0.05% trifluoroacetic acid (TFA) aqueous solution (A) and methanol (B). The assay was carried out at a flow rate of 1 mL/min at 25 °C with detection at 260 nm. Cytidine, uridine, adenosine and guanosine showed good linearity in the ranges of 4.02-603 μg/mL (r² = 0.9998), 9.38-1407 μg/mL (r² = 0.9999), 80.6-8060 μg/mL (r² = 0.9999) and 2.10-630 μg/mL (r²= 0.9987) with average recoveries of 97.2%, 94.5%, 98.6% and 108.6%, respectively. The contents of cytidine, uridine, adenosine and guanosine in different Carthamus tinctorius L. and Safflower injection were significantly different. This is the first report on the quantitative determination of nucleosides in Carthamus tinctorius L. and Safflower injection.

Key words: Carthamus tinctorius L., Carthamus tinctorius L., Safflower injection, Safflower injection, Nucleoside, Nucleoside, High-performance liquid chromatography, High-performance liquid chromatography

CLC Number:

R931.5

Supporting:

Fei-Ran Zhou, Ming-Bo Zhao, Peng-Fei Tu^*. Simultaneous determination of four nucleosides in Carthamus tinctorius L.
and Safflower injection using high-performance liquid chromatography [J]. .

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Simultaneous determination of four nucleosides in Carthamus tinctorius L.
and Safflower injection using high-performance liquid chromatography

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