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Antileukemic activity of jaspolide B, an isomalabaricane-type triterpene from marine sponge Jaspis sp. on human promyeloleukemic HL-60 cells

Min Li, Shao-Yin Wei, Sheng-An Tang, Wen-Han Lin, Jing-Rong Cui*   

  1. The State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100083, China
  • Received:2007-10-16 Revised:2008-01-10 Online:2008-03-15 Published:2008-03-15
  • Contact: Jing-Rong Cui*

Abstract: The antiproliferative activity and underlying mechanisms of jaspolide B, an isomalabaricane-type triterpene, isolated from the sponge Jaspis sp., were investigated using human promyeloleukemic HL-60 cells. Jaspolide B arrested HL-60 cells in the G2/M phase of the cell cycle and induced apoptosis of HL-60 cells in a dose- and time-dependent manner. Moreover, the poly (ADP-ribose) polymerase (PARP) protein was cleaved by jaspolide in a dose- and time-dependent way. Jaspolide B with an IC50 value of 0.61 μmol/L was found to be comparable efficacy as that of paclitaxel (IC50: 0.78 μmol/L). These results implicate the potential of jaspolide B as a promising anticancer agent in chemotherapy of leukemia by arresting cell cycle progression at G2/M phase and triggering apoptosis.

Key words: Jaspolide B, Jaspolide B, HL-60, HL-60, Cell cycle, Cell cycle, Apoptosis, Apoptosis

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