Abstract: Aim To establish an RP-HPLC method for determination of content of 1-phenylpropanol in its raw material and preparations. Methods Chromatography was carried out on a Dikma Diamonsil^TM ODS column, using a mobile phase of methanol-water (55:45) with a flow rate at 1.0 mL·min^-1. The detection wavelength was 258 nm. Results Under the chromatographic condition, the peaks of 1-phenylpropanol and its related impurities separated completely; non-interference between the principal agent and adjuvants in preparations was performed. The calibration curve was linear over the range of 250 - 750 μg·mL^-1 with the correlation coefficient of 0.999 9. The average recovery was 100.2% (RSD = 1.35%). Conclusion This method is simple, rapid, accurate, specific, and can be used to detect the content and related compounds of phenylpropanol in its raw material and preparations.

Key words: phenylpropanol, phenylpropanol, RP-HPLC, RP-HPLC, determination, determination