Application of a quantitative 1H NMR method for rapid extraction and determination of the content of paeonol in Cynanchum paniculatum

doi:10.5246/jcps.2020.06.040

Abstract

Abstract:

Cynanchum paniculatum (Bunge) Kitagawa is usually used as an herbal medicine for treating many diseases. Paeonol is the main active component, and its content is the key indicator for quality control of C. paniculatum. In the present study, we developed a rapid, accurate and precise method for quantitation of paeonol in C. paniculatum using ¹H NMR spectra. The deuteratedsolvent of methanol-d₄ enabled satisfactory separation of the signals to be integrated in ¹H NMR spectrum. H-6 (δ 7.78) of¹H NMR spectrum of C. paniculatum was selected as the feature signal for quantitation, and trimesic acid (TMA) was selected as an internal standard. Validation of the quantitative method was performed in terms of linearity, specificity, repeatability and stability. This is the first time to report quantitative ¹H NMR(qHNMR) applied to determine the content of paeonol in C. paniculatum and showed a wider linearity range than the reported quantitation of paeonol in others. The simple extraction of paeonol from C. paniculatum was rapid and will prompt the application of the developed method. This work implied that qHNMR represented a feasible alternative to HPLC-based methods for quantitation of paeonol in C. paniculatum, and it was suitable for the quality control of C. paniculatum.

Key words: Cynanchum paniculatum, Quantitative 1H NMR, Paeonol, Quantitative determination

CLC Number:

R917

Supporting:

Xuejiao Li, Jianwei Dong, Xiu Gao, Guijun Li, Junyou Shi, Yanqing Zhang. Application of a quantitative 1H NMR method for rapid extraction and determination of the content of paeonol in Cynanchum paniculatum[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(6): 422-430.

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