Abstract: A stability-indicating HPLC method is described for the determination of nateglinide in a new tablet formulation. Chromatographic separation was achieved using a mobile phase consisting of acetonitrile, methanol and phosphate buffer(70:4:20, v/v/v, pH 6.6) at a flow rate of 1.0 mL·min^-1 on a Hypersil BDS C18 column. Detection was carried out using a UV detector at 216 nm. The detector response was linear in the range of 4.94-49.4 μg·mL^-1 for nateglinide. The described method was validated with respect to selectivity, precision, accuracy and limits of detection and quantitation.

Key words: HPLC, HPLC, Nateglinide, Nateglinide, Tablet formulation, Tablet formulation