Abstract: Quantitative determinations of streptomycin, benzylpeincillin and oxytetracycline by the first derivative differential pulse polarography (FDDPP), and of chloralnphenicol, kanamycin and ribostamycin by the second derivative differential Pulse polarography (SDDPP) were respectively studied. They showed very good linear relationship to the polarographic Peak heights (amplitudes) in the concentration ranges of 200~700 μg·ml^-1 (streptomycin), 35~210 μg·ml^-1 (benzylpenicillin), 10~60 μg·ml^-1 (oxytetracycline), 32~192 μg·ml^-1 (chloramphenicol), 150~900 μg·ml^-1 (kanamycin), and 80~480 μg·ml^-1 (ribostamycin) (p<0.01). The peak potentials and detection limits of streptomycin, benzylpenicillin, oxytetracycline, chloramphenicol, kanamycin and ribostamycin are 0. 100V, 5.6×10^-8 mol·L^-1, -1.400V, 8.0×10^-9 mol·L^-1, -1.536V, 6.9×10^-7 mol·L^-1,-0.060V, 6.0×10^-9 mol·L^-1, -0.370V, 4.6×10^-8 mol·L^-1, and -0.105V, 7.2×10^-9 mol·L^-1, respectively. This method is simple, rapid and sensitive, and the results are accurate.

Key words: Derivative differential pulse polarography(DDPP), Streptomycin, Benzylpenicillin, Oxytetracycline, Chloramphenicol, Kanamycin, Ribostamycin