Abstract: Six factors and 10 levels of each factor were selected by using the (uniform design method( with the aid of the computer for preparing APS liposomes. The optimal procedure for preparing APS liposomes was established and it can suit the large scale production in a pharmaceutical factory. The shelf-life of APS liposomes at 20 ºC is 1.46 years. Diameters of the vesicles (>90% ) in APS liposomes are less than 1 μm, and the system is stable. At 40 ºC the diameters of vesicles were not changed in three months. Pharmacological experiments revealed that APS liposomes exerted a strong immunoenhancement in mice. Studies in this paper established a foundation for the production and the clinical application of APS liposomes.

Key words: APS liposomes, Stability, Shelf-life, Immunoen