Ela tablets improve sexual performance in mice with erectile dysfunction caused by repeated restraint stress

doi:10.5246/jcps.2021.11.076

Journal of Chinese Pharmaceutical Sciences ›› 2021, Vol. 30 ›› Issue (11): 883-894.DOI: 10.5246/jcps.2021.11.076

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Ela tablets improve sexual performance in mice with erectile dysfunction caused by repeated restraint stress

Zeyu Gao¹^,²^,^#, Jun Chen³^,⁴^,^#, Ayinuer Reheman³^,⁴^,⁵, Yi Sun¹^,², Tao Wu³, Xirali Tursun⁴, Xiaoping Pu¹^,²^,^*(), Haji Akber Aisa³^,^*(), Xin Zhao¹^,²^,^*()

1 State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
2 Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
3 Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, Xinjiang, China
4 University of Chinese Academy of Sciences, Beijing 100039, China
5 College of Traditional Uyghur Medicine, Xinjiang Medical University, Urumqi 830011, Xinjiang, China

Received:2021-04-21 Revised:2021-05-18 Accepted:2021-07-20 Online:2021-11-28 Published:2021-11-28
Contact: Xiaoping Pu, Haji Akber Aisa, Xin Zhao
About author:
# Zeyu Gao and Jun Chen contributed equally to this work.

Abstract

Abstract:

Ela tablets contain an optimized extract of a Chinese herbal prescription. The original prescription has been used to treat erectile dysfunction (ED), while the detailed indications and specific treatment mechanisms of Ela tablets remain unclear. In the present study, we aimed to investigate whether Ela tablets could protect mice from ED caused by repeated restraint stress and to explore the possible mechanisms. Mice were restrained daily in centrifuge tubes with venting holes for 14 consecutive days and administered Ela tablets at 1 h before restraining. On the 14^th night, mating experiments were conducted. Thereafter, blood, testicular, and penile samples were collected. Serum testosterone levels were measured using a kit. Testicular tissue morphology was observed by H&E staining. The PDE5 expression in the corpus cavernosum was measured by Western blotting analysis. The results indicated that Ela tablets significantly shortened the sexual arousal time, increased the number of sexual encounters, maintained the normal morphology of testicular tissue, increased the serum testosterone levels, and decreased the PDE5 expression in the corpus cavernosum. Our data showed that Ela tablets elicited protective effects on the sexual ability of mice exposed to repeated restraint stress. Moreover, the underlying mechanism was related to the preservation of testicular tissue morphology, maintenance of testosterone levels, and inhibition of penile PDE5 expression.

Key words: Ela tablets, Erectile dysfunction, Repeated restraint stress

Supporting:

Zeyu Gao, Jun Chen, Ayinuer Reheman, Yi Sun, Tao Wu, Xirali Tursun, Xiaoping Pu, Haji Akber Aisa, Xin Zhao. Ela tablets improve sexual performance in mice with erectile dysfunction caused by repeated restraint stress[J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(11): 883-894.

Figures/Tables 9

Table 1. Quality standard for Ela tablet production.

Table 2. Herbs used for preparing Ela tablets.

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Ela tablets improve sexual performance in mice with erectile dysfunction caused by repeated restraint stress

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