Extractum trametes robiniophila murr protects murine podocyte via actin cytoskeleton stabilization

doi:10.5246/jcps.2021.03.018

Journal of Chinese Pharmaceutical Sciences ›› 2021, Vol. 30 ›› Issue (3): 230-238.DOI: 10.5246/jcps.2021.03.018

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Extractum trametes robiniophila murr protects murine podocyte via actin cytoskeleton stabilization

Xuan Luo¹, Qing Du¹, Haiyan Zhao¹, Wenhai Fan¹, Xin Gao², Xia Gao³^,^*()

1 Department of Pediatric, People’s Hospital of Gansu province, Lanzhou, Gansu 730000, China
2 Department of Intensive care center, People’s Hospital of Gansu province, Lanzhou, Gansu 730000, China
3 Nephrology department, Guangzhou Women and Children’s Medical Center, Guangzhou city 510623, China

Received:2020-07-29 Revised:2020-08-12 Accepted:2020-11-06 Online:2021-03-29 Published:2021-03-29
Contact: Xia Gao

Abstract

Abstract:

The Chinese medicine Extractum trametes robiniophila murr (ETRM) is the extract of a type of fungus. Recent studies have suggested that ETRM efficiently improves the effacement of podocyte foot processes in adriamycin (ADR)-induced nephrotic rats. In the present study, we aimed to assess whether ETRM modulated the actin rearrangements of podocytes and involved signaling molecules, including α-actinin-4 and nephrin. Podocytes were treated with ADR (0.5 μmol/L), ADR (0.5 μmol/L) + dexamethasone (Dex) (1 μmol/L), ADR (0.5 μmol/L) + ETRM (10 mg/mL). The F-actin in the podocytes was stained by fluorescent phallotoxins and observed by confocal microscopy. The expression levels of α-actinin-4 and nephrin were tested by real-time PCR and Western blotting analysis. The administration of ETRM could significantly prevent ADR-treated podocytes from actin rearrangement. Both ETRM and Dex could stabilize podocyte actin cytoskeletons. Moreover, α-actinin-4 might act as a potential target for ETRM functionality in podocyte actin rearrangements. However, pretreatment with ETRM could not inhibit the up-regulation of nephrin as a result of ADR treatment. ETRM could improve the cytoskeleton stability in ADR-induced actin rearrangement of podocytes via regulating α-actinin-4 expression.

Key words: Actin rearrangements, Extractum trametes robiniophila murr, Foot processes, Adriamycin

Supporting:

Xuan Luo, Qing Du, Haiyan Zhao, Wenhai Fan, Xin Gao, Xia Gao. Extractum trametes robiniophila murr protects murine podocyte via actin cytoskeleton stabilization[J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(3): 230-238.

Figures/Tables 3

References 19

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Extractum trametes robiniophila murr protects murine podocyte via actin cytoskeleton stabilization

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