Determination of β-amyloid peptides in vitro aggregation process by electrochemistry

doi:10.5246/jcps.2020.03.017

Abstract

Abstract:

An impedimetric sensor (EA/mAb/CeZnO/GCE) for detecting β-amyloid peptides (Aβ) was successfully constructed by synthesizing ceria doped zinc oxide (CeZnO) nanoparticles to modify the glassy carbon electrode (GCE) for immobilizing the antibody mAb, and blocking the unbound carboxyl sites with ethanolamine (EA). The sensor exhibited a linear detection range of 10–100 pM and a detection limit of 1.7 pM (S/N = 3), and the relative standard deviations (RSDs) of the inter-electrode and intra-electrodes were less than 3.0%. After it was stored at 4 °C for 2 d, its impedance value was 101.6% of original impedance response. The sensor was used to measure the remaining content of Aβ incubated in vitro. The results showed that after incubation for 48 h and 60 h, the content of aggregated Aβ (oligomers and fibers) almost unchanged, reaching about 90%. This method had the advantages of time-saving, simple procedure, economical, excellent conductivity, good reproducibility and stability.

Key words: Electrochemical sensor, Electrochemical impedance spectroscopy, β-Amyloid peptides

CLC Number:

R917

Supporting:

Sufang Zhang, Cong Li, Ying Zhang, Juncheng Zou, Yinzhu Cui, Xiaomei Ling. Determination of β-amyloid peptides in vitro aggregation process by electrochemistry[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(3): 199-205.

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