Lanthanum chloride or citrate is absorbed mainly via M cells in gastrointestinal tracts with lanthanum phosphates as the transformed species

doi:10.5246/jcps.2018.08.056

Abstract

Abstract:

本工作以氯化镧(LaCl₃)和柠檬酸镧(LaCit)为代表性化合物, 对稀土在消化道内的物种存在形式及吸收转运机制进行了研究和探讨。研究采用了人工胃液和肠液模拟体内消化道环境。电感耦合等离子体质谱(ICP-MS)检测结果表明, 大于99.9%的La发生了沉淀。散射光粒度(DLS)分析结果显示, 这些微粒的平均粒径由200 nm (孵育2 h)增大到600 nm左右(孵育24 h), 这表明微粒之间发生了聚集。傅立叶变换红外光谱(FTIR)研究表明, 稀土微粒的主要成分为稀土磷酸盐。为研究稀土在肠道中的吸收转运机制, 以LaCit (2或100 mg/kg/day)对小鼠灌胃给药7天, 取小肠的普通肠段和派氏结进行了ICP-MS分析。派氏结中的La含量明显高于普通肠段, 这提示稀土微粒可能主要由派氏结上淋巴滤泡上皮中的M细胞吞噬转运。此外, 建立了Caco-2细胞单培养模型和 Caco-2/Raji B 细胞共培养模型分别模拟小肠上皮和滤泡上皮。结果显示, Caco-2/Raji B 细胞共培养模型转运的La含量显著高于Caco-2细胞单培养模型(大约60倍左右), 且 Caco-2 细胞单层透过的镧的量极低, 这说明M细胞是吸收转运磷酸镧的主要途径。以上结果表明氯化镧和柠檬酸镧在胃肠道主要以磷酸镧微粒形式通过M细胞进行转运吸收。本研究将为稀土在农业和医学中的合理应用提供科学依据。

Key words: 氯化镧, 柠檬酸镧, 磷酸镧, 人工肠液, 派氏结, M细胞, Caco-2 细胞单培养模型, Caco-2/Raji B 细胞共培养模型, 吸收转运

CLC Number:

R916.3

Supporting:

Huixia Huang, Huixue Liu, Xiaojie Ma, Hui Guan, Xiaogai Yang. Lanthanum chloride or citrate is absorbed mainly via M cells in gastrointestinal tracts with lanthanum phosphates as the transformed species[J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(8): 553-564.

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