Genome-wide identification of long noncoding RNAs in myocyte differentiation

doi:10.5246/jcps.2017.06.046

Journal of Chinese Pharmaceutical Sciences ›› 2017, Vol. 26 ›› Issue (6): 423-431.DOI: 10.5246/jcps.2017.06.046

• Original articles • Previous Articles Next Articles

Genome-wide identification of long noncoding RNAs in myocyte differentiation

Qin Yang¹, Dong Li¹, Chao Feng¹, Yi Fan¹, Hua Li², Quan Du^1*

1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
2. Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China

Received:2017-04-04 Revised:2017-04-25 Online:2017-06-29 Published:2017-05-06
Contact: Tel.: +86-010-82805780, E-mail: quan.du@pku.edu.cn
Supported by:
Beijing Natural Science Foundation (Grant No. 2171001), the National Natural Science Foundation of China (Grant No. 31571403) and the National High-tech R&D Program of China (Grant No. 2014AA021103).

Abstract

Abstract:

Taking advantage of high-throughput sequencing technology, a large number of long noncoding RNAs (lncRNAs) have been recently characterized. Emerging evidence suggests that they play critical roles in diverse biological processes, including the differentiation of skeletal muscle cells. As previous studies have focused mainly on the polyadenylated lncRNAs, the involvement of polyA-minus lncRNAs in myocyte differentiation remains largely unexplored. To this aspect, their expression and regulation were examined in the present study. During the course of myocyte differentiation of C2C12 cells, polyA-minus RNAs were isolated and analyzed by RNA-seq. In addition to identifying 904 novel polyA-minus lncRNAs, their temporal expression was further characterized in this process. For many lncRNAs, differentiation-specific profiles were revealed. Based on their unique expression profiles, these lncRNAs were grouped into nine regulation categories. Taken together, our study greatly contributed to the identification of a dynamic regulation of polyA-minus lncRNAs, and clarified their potential roles in myocyte differentiation.

Key words: LncRNA, RNA-Seq, Myocyte differentiation

CLC Number:

R963

Supporting:

Qin Yang, Dong Li, Chao Feng, Yi Fan, Hua Li, Quan Du. Genome-wide identification of long noncoding RNAs in myocyte differentiation[J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(6): 423-431.

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Genome-wide identification of long noncoding RNAs in myocyte differentiation

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