Preparation and characterization of mesoporous silica nanoparticles with enlarged pores capped with crosslinked PEI

doi:10.5246/jcps.2015.11.091

Journal of Chinese Pharmaceutical Sciences ›› 2015, Vol. 24 ›› Issue (11): 712-720.DOI: 10.5246/jcps.2015.11.091

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Preparation and characterization of mesoporous silica nanoparticles with enlarged pores capped with crosslinked PEI

Lu Sun^1,2, Yujie Liu^1,2, Zhenzhen Yang^1,2, Xianrong Qi^1,2,3*

1. Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery System, Peking University Health Science Center, Beijing 100191, China
2. Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
3. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Sciences Center, Beijing 100191, China

Received:2015-05-17 Revised:2015-06-20 Online:2015-11-20 Published:2015-07-20
Contact: Tel.: 86-10-82801584, E-mail: qixr@bjmu.edu.cn
Supported by:
National Natural Science Foundation of China (Grant No. 81273454 and 81473156), Beijing Nature Science Foundation (Grant No. 7132113), Doctoral Foundation of the Ministry of Education (Grant No. 20130001110055), National Key Basic Research Program (Grant No. 2013CB932501).

Abstract

Abstract:

The epidemiological statistics reveals the striking patterns of cancer in women and highlights the need for novel therapeutic strategies. In this work, mesoporous silica nanoparticles (MSNs) as representative of inorganic nanoparticles were prepared for loading siRNA that plays a role of gene silencing to treat breast carcinoma (MCF-7) cells. The critical processes of synthesis were optimized for the nanoparticles with desired quality attributes that have the enlarged pores for elevated loading capacity.After siRNA loading into mesoporous, crosslinked-polyethylenimine was employed as the cap to coat the enlarged MSN pores and protect the cargo from leakage. The elevated quantity of siRNA (35 μg siRNA/mg MSNs) were loaded in the MSNs. The as-synthesized MSNs were further evaluated on MCF-7 cells in vitro and shown negligible cytotoxicity.As expected, the siRNA loaded in the as-synthesized MSNs was readily internalized into MCF-7 cells and displayed 420 times higher intake than that of naked siRNA. The MSNs may be exploited to become an effective siRNA cell delivery strategy and further studied for the anti-tumor efficacy.

Key words: Mesoporous silica nanoparticle, Preparation, Polyethylenimine, MCF-7 cells

CLC Number:

R943

Supporting:

Lu Sun, Yujie Liu, Zhenzhen Yang, Xianrong Qi. Preparation and characterization of mesoporous silica nanoparticles with enlarged pores capped with crosslinked PEI[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(11): 712-720.

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Preparation and characterization of mesoporous silica nanoparticles with enlarged pores capped with crosslinked PEI

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