Qualitative and quantitative analysis of four different polarity fractions from Huang-Lian-Jie-Du-Decoction by HPLC-DAD-ESI-MS/MS and their related neuroprotective effects

doi:10.5246/jcps.2013.04.048

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Qualitative and quantitative analysis of four different polarity fractions
from Huang-Lian-Jie-Du-Decoction by HPLC-DAD-ESI-MS/MS and their related neuroprotective effects

Yang Yang, Haiyu Zhao, Hongjie Wang, Jianfang Song, Jian Yang, Nan Si, Baolin Bian^*

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

Received:2013-01-06 Revised:2013-02-22 Online:2013-07-10 Published:2013-07-10
Contact: Baolin Bian*

Abstract

Abstract: Huang-Lian-Jie-Du-Decoction (HLJDD) has been widely used for the treatment of Alzheimer’s disease (AD) in clinic. However, the relationship between its chemical profile and neuroprotective bioactivity was not clearly clarified yet. In present study, the water extract of HLJDD and subsequent three polarity fractions divided by different reagents were investigated. A total of 17 chromatographic peaks were confirmed by comparison with standards and their UV, MS spectra. Among them, 11 major compounds were determined by HPLC-DAD method with good linear regression relationship (r², 0.9994-0.9999), precisions (inter-day precision RSD, 0.79%-1.07%; intra-day precision RSD, 1.59%-2.10%), repeatability (RSD, 1.66%-3.67%), stability (RSD, 1.26%-4.77%) and recovery (95.24%-105.41%, RSD, 0.29%-2.69%). Furthermore, PC12 cells and primary neurons cells were used for the neuroprotective effective assessment of aforementioned four samples from HLJDD. The total aqueous extract and n-butanol extract of HLJDD presented more significant effects than the other two parts. According to their quality and quantity determination results, iridoids and alkaloids have a positive correlation with the neuroprotective effectiveness of HLJDD.

Key words: Huang-Lian-Jie-Du-Decoction, Qualitative and quantitative analysis, Neuroprotective effects

CLC Number:

R284.1

Supporting:

Yang Yang, Haiyu Zhao, Hongjie Wang, Jianfang Song, Jian Yang, Nan Si, Baolin Bian^*. Qualitative and quantitative analysis of four different polarity fractions
from Huang-Lian-Jie-Du-Decoction by HPLC-DAD-ESI-MS/MS and their related neuroprotective effects[J]. , DOI: 10.5246/jcps.2013.04.048.

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Qualitative and quantitative analysis of four different polarity fractions
from Huang-Lian-Jie-Du-Decoction by HPLC-DAD-ESI-MS/MS and their related neuroprotective effects

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