Acute toxicity and genotoxicity evaluation of hyperoside extracted from Abelmoschus manihot (L.) Medic

doi:10.5246/jcps.2012.05.063

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Acute toxicity and genotoxicity evaluation of hyperoside extracted from Abelmoschus manihot (L.) Medic

Guo Ai, Zhengming Huang^*, Dewen Wang, Haiting Zhang

1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
2. Department of Pharmacy, 302 Hospital of PLA, Beijing 100039, China
3. Center for New Drugs Evaluation of Shandong University, Jinan 250100, China

Received:2012-05-18 Revised:2012-07-20 Online:2012-09-15 Published:2012-09-15
Contact: Zhengming Huang*

Abstract

Abstract:

To further assess hyperoside as a potential new anti-hepatitis B virus (HBV) drug, the safety of hyperoside extracted from Abelmoschus manihot (L.) Medic was evaluated by testing its acute toxicity and mutagenic risk. To test the acute toxicity of hyperoside, we determined the median lethal dose (LD₅₀) in mice. Forty healthy BALB/c mice (20 per sex) were administered a single oral dose of 5000 mg/kg hyperoside via the intragastrical route. The number of animals poisoned and died was noted daily for 14 consecutive days. All animals survived and appeared active and normal, indicating that the LD₅₀ of hyperoside was more than 5000 mg/kg. Potential genotoxicity of hyperoside was investigated using a bacterial reverse mutation assay (Ames test), a chromosome aberration test in Chinese hamster lung (CHL) fibroblasts, and an in vivo micronucleus test in rat bone marrow cells. In the bacterial reverse mutation assay, we observed no increases in the number of revertant colonies at any concentrations of hyperoside regardless of metabolic activation (S9) in all tester strains (TA97, TA98, TA100 and TA102) compared to the vehicle control (P>0.05). Hyperoside did not cause significant structural aberration in CHL cells in the presence or absence of S9 (P>0.05). The micronuclei rates of mice bone marrow cell in all groups showed no significant difference when compared with the negative control (P>0.05). In summary, hyperoside showed no genotoxicity in our experimental conditions.

Key words: Hyperoside, Acute toxicity, Genotoxicity

CLC Number:

R965.3

Supporting:

Guo Ai, Zhengming Huang^*, Dewen Wang, Haiting Zhang. Acute toxicity and genotoxicity evaluation of hyperoside extracted from Abelmoschus manihot (L.) Medic[J]. , DOI: 10.5246/jcps.2012.05.063.

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Acute toxicity and genotoxicity evaluation of hyperoside extracted from Abelmoschus manihot (L.) Medic

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