Loading...
http://jcps.bjmu.edu.cn

Table of Content

    23 May 2014, Volume 23 Issue 5
    Contents
    Graphical contents list
    Journal of Chinese Pharmaceutical Sciences
    2014, 23(5):  275-278. 
    Asbtract ( 215 )   HTML ( 4)   PDF (510KB) ( 245 )  
    References | Related Articles | Metrics
    Original articles
    Detection of microRNA expressions in tissues and exfoliative cells reveals the potential role of miR-203 in oral lichen planus
    Zhendong Feng, Wen Shi, Zhigang Cai, Hong Hua, Demin Zhou
    2014, 23(5):  279-286.  DOI: 10.5246/jcps.2014.05.038
    Asbtract ( 329 )   HTML ( 2)   PDF (1736KB) ( 440 )  
    References | Related Articles | Metrics
    Oral lichen planus (OLP) is a chronic inflammatory disorder and premalignantlesion, of which the mechanisms are still obscure. In the present study, the expression levels of miR-96/182/183 cluster, miR-203, miR-375, and miR-769-5p in both tissues and exfoliative cells of OLP patients as well as healthy volunteers were detected, differentially expressed miRNAs were identified and their correlation with OLP was evaluated by a biplot method. Experimental results show that miR-203 is significantly up-regulated in patient lesion tissues in comparison to volunteer mucosa tissues. Moreover, the contradictory insignificant expression changes of miR-203 as well as miR-96/182/183 cluster in comparisons of exfoliative cell samplessuggest that different cell compositions in OLP lesion have distinct miRNA regulation, which accords with the histological heterogeneity of OLP. Finally, biplot analyses indicate the expression of miR-203 and miR-96/182/183 cluster are positively correlated in patient lesions. These results provide miR-203 as a molecular indicator of heterogeneity of OLP, and also a potential diagnostic biomarker or therapeutic target that deserves further studies.
    Development of targeted sunitinib plus vinorelbine liposomes modified with DSPE-PEG2000-pemetrexed conjugate and the inhibitory effect toresistant breast cancer in vitro
    Jifeng Shi, Ruijun Ju, Mengge Sun, Xiuying Li, Yao Zhao, Fan Zeng, Wanliang Lu
    2014, 23(5):  287-294.  DOI: 10.5246/jcps.2014.05.039
    Asbtract ( 444 )   HTML ( 3)   PDF (1012KB) ( 346 )  
    References | Related Articles | Metrics
    Multidrug resistance (MDR) of breast cancer is a major cause of failure in chemotherapy. In the present study,a distearoylphosphatidyl ethanolamine-polyethylene glycol-pemetrexed (DSPE-PEG2000-PMT) conjugate was synthesized from DSPE-PEG2000-NH2 and pemetrexed, and targeted sunitinib plus vinorelbine liposomes were developed by modifying DSPE-PEG2000-PMT onto the surface of liposomes to overcome the MDR of breast cancer. The synthesized DSPE-PEG2000-PMT was confirmed to be consistent with the target product by matrix-assisted laser desorption/ionization time of flight massspectrometry (MALDI-TOF-MS). The concentrations of sunitinib and vinorelbine were measured simultaneously by highperformance liquid chromatography (HPLC). The analysis was performed on an ODS column at 30 °C at a wavelength of 215 nm with the mobile phase consisting of acetonitrile, 0.05 M KH2PO4 (pH 3.5) and triethylamine (35:65:0.3, v/v/v). The limits of detection for sunitinib and vinorelbine were 25 ng/mL and 5 ng/mL, respectively, and the limits of quantification for both drugs were 0.25 µg/mL. Two drugs were linearly correlated in the range of 0.5–25.0 µg/mL. For varying types of liposomes, the encapsulation efficiencies were >90%; the particle sizes were approximately 90 nm, and zeta potentials were slightly negative. The inhibitory effects were evaluated in the resistant breast cancer MCF-7/Adr cells. The results revealed that targeted sunitinib plus vinorelbine liposomes exhibited the strongest inhibitory effect to the resistant MCF-7/Adr cells among the varying formulations. Targeted coumarin liposomes were used as a fluorescent probe to evaluate the targeting effect to resistant breast cancer MCF-7/Adrcells. The results demonstrated that the targeted coumarin liposomes displayed the highest cellular uptake compared tonon-targeted formulations. In conclusion, the targeted sunitinib plus vinorelbine liposomes represented a novel type of nano-formulations, which could accumulate in the resistant breast cancer cells, thereby inhibiting proliferation of the resistant cancer cells. Accordingly, the targeted sunitinib plus vinorelbine liposomes may provide a new strategy for circumventing the drug resistance in the resistant breast cancer.
    Intervention of multiple pathways by multiple active components provides potent protection against cerebral ischemia injury
    Jingliang Zhang, Tao Hu, Xiaoyan Liu, Yuanjun Zhu, Yinye Wang
    2014, 23(5):  295-301.  DOI: 10.5246/jcps.2014.05.040
    Asbtract ( 373 )   HTML ( 1)   PDF (882KB) ( 384 )  
    References | Related Articles | Metrics
    To explore the effect of multiple pathway intervention in acute cerebral ischemia injury, we prepared a medicine formula (formula 2) consisting of ginsenosides, pueraria flavonoids, ophiopogonis and borneol as a tool medicine. The effects of formula 2 and its components on PC12 cell viability and potential pathway were investigated, and the influence of this formula on venous thrombosis and platelet aggregation was also assessed, then the effect of formula 2 on middle cerebral artery occlusion (MCAO) reperfusion was observed in rats. Formula 2 markedly enhanced the cell viability, which was stronger than that of each individual component. Formula 2 significantly inhibited the NO production in PC12 cells induced by H2O2, and this effect was also stronger than that of each individual component. Moreover, formula 2 enhanced the SOD activity, and the effect was stronger than that of ginsenosides. In addition, formula 2 reduced the MDA content, and this effect was stronger than that of ophiopogonins. In vivo, formula 2 showed potent inhibitory effects on platelet aggregation and venous thrombosis. Furthermore, formula 2 (single dose, s.c.) significantly reduced the infarct volume and neurobehavioral scores in MCAO reperfusion rats. Take together, our results suggests that formula 2 has powerful ability of inhibiting the ischemia/reperfusion injury, and this effect might be attributed to its simultaneous intervention in the cascade reaction of neuronal injury via multiple pathways contributed by multiple components during cerebral ischemia/reperfusion.
    Characterization of two degradation products of Azilsartan formed during the micronization process and development of a method to control the degradation
    Lipeng Sui, Ridong Li, Bo Song, Moyi Liu
    2014, 23(5):  302-305.  DOI: 10.5246/jcps.2014.05.041
    Asbtract ( 499 )   HTML ( 2)   PDF (726KB) ( 562 )  
    References | Related Articles | Metrics
    The degradation of Azilsartan in the micronization process was studied and a degradation control method was developed. In the micronization process of Azilsartan, two degradation products (DP-1 and DP-2) were detected by HPLC. DP-1 is a known impurity but DP-2 has not been reported before. The structures of the two degradation products were identified by NMR and MS analysis. The formation of DP-1 and DP-2 can be controlled by changing the micronization process.
    A new indole alkaloid from Nauclea officinalis (Pierre ex Pitard) Merr. et Chun
    Xiaoxue Pi, Kehui Xie, Guangzhong Tu, Tianzhi Cai, Rong Yang, Qiong Wu, Hongzheng Fu
    2014, 23(5):  306-310.  DOI: 10.5246/jcps.2014.05.042
    Asbtract ( 400 )   HTML ( 0)   PDF (777KB) ( 382 )  
    References | Related Articles | Metrics
    From the aerial parts of Nauclea officinalis (Pierre ex Pitard) Merr. et Chun, one new indole alkaloid paratunamide E (1)has been isolated together with six known indole alkaloids, paratunamide A (2), nauclealotide C (3), nauclealotide A (4), vincosamide (5), strictosamide (6) and naucleamide F (7). Naucleamide F (7) was isolated from Nauclea officinalis for the first time. All of the seven compounds above were elucidated by spectroscopic methods including 1 D and 2 D NMR spectroscopic analyses.
    Biotransformation of methyleugenol by rat hepatic microsomes
    Guiyun Cao, Xiuwei Yang
    2014, 23(5):  311-316.  DOI: 10.5246/jcps.2014.05.043
    Asbtract ( 339 )   HTML ( 1)   PDF (799KB) ( 683 )  
    References | Related Articles | Metrics
    Methyleugenol (1), one of the main bioactive constituents of the seeds of Myristica fragrans Houtt. (family: Myristicaceae),was incubated with rat hepatic microsomes from rats pretreated with sodium phenobarbital. Eight biotransformation products named (R)-1'-methoxymethyleugenol (2), 3-(3,4-dimethoxyphenyl)-l-methoxyprop-2-ene (3), cis-3,4-dimethoxycinnamyl acetate (4), trans-3,4-dimethoxycinnamyl acetate (5), (R)-1'-hydroxymethyleugenol (6), trans-3,4-dimethoxycinnamyl alcohol (7), cis-3,4-dimethoxycinnamyl alcohol (8), and (R)-3-(3,4-dimethoxyphenyl)-propane-1,2-diol (9) were obtained and their structures were elucidated by NMR and MS data analysis and by comparison with the previously reported data. The biotransformation of 1 may provide valuable information for the use of methyleugenol. The NMR data of compounds 4 and 6 were reported for the first time.
    Comparative study on different derivatization procedures for analysis of recombinant human erythropoietin by capillary electrophoresis with laser-induced fluorescence detection
    Xia Yang, Nannan Pang, Xiaofang Fu, Hongfeng Yin, Yiping Liao, Huwei Liu
    2014, 23(5):  317-323.  DOI: 10.5246/jcps.2014.05.044
    Asbtract ( 330 )   HTML ( 0)   PDF (860KB) ( 326 )  
    References | Related Articles | Metrics
    Human erythropoietin (hEPO), an endogenous glycoprotein, plays a fundamental role in erythropoiesis controlling the formation of red blood cells. Production of recombinant human erythropoietin (rhEPO) has made it possible for its abuse in competitive sports. In this work, pre-capillary and on-capillary derivatization by 5-furoylquinoline-3-carboxaldehyde (FQ) and fluorescein isothiocyanate (FITC) for the detection of rhEPO by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) were compared. FQ pre-capillary labeling improves sensitivity but degrades the glycoforms separation due to the inhomogeneity of the reaction products from multiple labeling. Compared with FITC pre-capillary derivatization with the excess fluorescent background, the on-capillary FQ derivatization method can provide shorter analysis time, lower background, and better selectivity. It is demonstrated that, through optimizing reaction conditions of FQ on-capillary derivatization, both high sensitivity and satisfactory resolution for the analysis of the glycoforms of rhEPO could be obtained. This method can be used for the glycoforms profiling and quality control of rhEPO. It may be used as a candidate method for fast screening in doping control analysis.
    Drug administration column
    The impact of drug administration in USA, Europe and Japan on the reform plan in China
    Xiaoyuan Zheng, Shixia Zhang, Song Yang, Yanyan Li, Bin Jiang
    2014, 23(5):  324-329.  DOI: 10.5246/jcps.2014.05.045
    Asbtract ( 355 )   HTML ( 1)   PDF (782KB) ( 438 )  
    References | Related Articles | Metrics
    It is critical to ensure the safety and efficacy of human drugs through drug administration. Drug administration has a long history in developed countries, such as USA, European Union and Japan, and has achieved great success. In this study, we summarized the important changes in the practice of drug administration in USA, European Union and Japan since 1990s. We also discussed how these changes and experience could be applied in the reform plan in drug administration in China Food and Drug Administration (CFDA). Our suggestions on future developmentofCFDA include improving post-market surveillance, advancing regulatory science, strengthening service-oriented regulation, and international cooperation.
    Short communications
    Participation of clinical pharmacist specialized in oncology in the treatment of a patient with advanced esophageal carcinoma complicated with hypertension: one case report
    Zeng Wang, Haiying Ding, Meiqin Yuan, Nengming Lin
    2014, 23(5):  330-334.  DOI: 10.5246/jcps.2014.05.046
    Asbtract ( 398 )   HTML ( 1)   PDF (755KB) ( 424 )  
    References | Related Articles | Metrics
    Clinical pharmacists specialized in oncology contributed to the safe and effective chemotherapy treatment for advanced esophageal carcinoma patients complicated with hypertension by participating in medication practice. Optimal therapeutic regimen was proposed, and pharmaceutical care and health education were provided based on the condition of each patient. By providing pharmaceutical care for cancer patients with their pharmacy knowledge, clinical pharmacists specialized in oncology can not only reduce the potential risks of chemotherapy, but also take into account of other accompanying diseases, thus improving the comprehensive treatment of patients. Furthermore, it deepened the understanding of the role of clinical pharmacy practice by patients and other medical workers. Involvement of clinical pharmacist in therapeutic practice could improve the outcome of pharmacotherapy.
    Application of recombinant enzymes in the production of human insulin
    Chunsheng Leng, Hang Cui, Shunan Li
    2014, 23(5):  335-337.  DOI: 10.5246/jcps.2014.05.047
    Asbtract ( 449 )   HTML ( 0)   PDF (809KB) ( 416 )  
    References | Related Articles | Metrics
    In the present study, the cleavage activities of both recombinant and bovine enzymes(trypsin and carboxy peptidase B) were determined using proinsulin fusion protein. The proteolysis products were analyzed with HPLC.We found that recombinant enzymes had stronger activities and produced fewer by-products. In addition, recombinant enzymes were more efficient in the production of human insulin than bovine enzymes.
    Editor profile
    Ah-Ng Tony Kong
    Journal of Chinese Pharmaceutical Sciences
    2014, 23(5):  338-339. 
    Asbtract ( 323 )   HTML ( 0)   PDF (585KB) ( 279 )  
    Related Articles | Metrics

    Prof. Ah-Ng Tony Kong is Distinguished Professor, Glaxo Endowed Chair in Pharmaceutics and Director of the Graduate Program in Pharmaceutical Sciences. He is Co-Leader of the Carcinogenesis and Cancer Prevention Program and Co-Director of the PK-PD facility Core at the Cancer Institute of New Jersey (CINJ). He serves on the Dean’s Budget and Policy (Executive) Committee, Ernest Mario School of Pharmacy since 2003. At the University level, he served on the Committee on Academic Planning and Review (CAPR; an advisory committee to the Executive Vice President of Academic Affairs, equivalent to chancellor) from 2007–2012. He was appointed the Interim Chair of PresidentCommittee on Academic Planning and Review (advisory committee to the Executive Vice President of Academic Affairs of Rutgers, and Interim Chancellor of Rutgers New Brunswick Campus) in 2012–2013. He continues to serve on the Committee on Academic Planning and Review, 2012–present. He is member of 13 editorial boards of international journals, oversea Editor-in-Chief of Journal of Chinese Pharmaceutical Sciencesfrom 2013, editor of Pharmaceutical Research (2004–present), and the editor in-chief of Current Pharmacology Reports (Springer; epub journal–first issue to appear January 2015). He has published more than 200 original research, review articles and book chapters in pharmacokinetics, pharmacodynamics, PK-PD modeling, drug metabolism, pharmacogenomics, cellular signaling, Nrf2-mediated anti-oxidative stress and anti-inflammatory responses, and cancer chemoprevention by dietary phytochemicals. He has been supported by the National Institutes of Health continuously since 1993, totaling more than $20 million. He has continued to serve on numerous NIH panels since 1998. He is the Editor-in-Chief,Current Pharmacological Reports (Springer, 2013), an Editor of Pharmaceutical Research, Associate Editor of Molecular Carcinogenesis and Life Sciences and member of Editorial Board of nine scientific journals such as Cancer Prevention Research, Carcinogenesis and Biopharmaceutics and Drug Disposition. He has directed the research of more than 40 doctoral students, postdoctoral associates, and visiting scholars. 

    Other
    Information from US FDA
    http://www.fda.com
    2014, 23(5):  340-342. 
    Asbtract ( 296 )   HTML ( 0)   PDF (564KB) ( 177 )  
    Related Articles | Metrics