Abstract: The effect of extract from leaves of Ginkgo biloba (EGb761) on the oxidation ofhuman serum low density lipoproteins (LDL) induced by Cu²⁺ in vitro was investigated . LDL waspretreated with EGb761 40, 80, or 160 mg·L^-1 for 1 h and then was oxidized by CuSO4 10 μmol·L^-1 for 10 h. It was found that after the oxidation of LDL induced by Cu²⁺, vitamin E (VE) in the oxidizedLDL (ox-LDL) was exhausted, while malondialdehyde (MDA) and lipofusion (LF) were markedlyincreased, the electrophoretic mobility of apolipoprotein B (apo B) was faster. EGb761 preveded thedecrease of VE and increases of MDA and LF in the ox-LDL, and slowed down the apo Belectrophoretic mobility in a dose-dependent manner. Therefore, EGb761 inhibits LDL oxidationinduced by Cu²⁺ in vitro.

Key words: Low density lipoproteins, Copper, Malondialdehyde, Vitamin E, Lipofusion, Ginkgo biloba