Sub-critical Column and Capillary Chromatography with Water as Mobile Phase and Flame Ionization Detection

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Sub-critical Column and Capillary Chromatography with Water as Mobile Phase and Flame Ionization Detection

Lu Feng, Li Ling, Sun Peng, Wu Yutian

College of Pharmacy, Second Military Medical University, Shanghai 200433

Received:2000-11-25 Revised:2001-01-20 Online:2001-03-15 Published:2001-03-15

Abstract

Abstract: A sub-critical chromatography (SubWC) with water as mobile phase and FID detection system is employed to separate several alcohols with high or medium polarity, with pure water as the eluent. The flow rate gets up to 50 μl·min^-1 for packed column (1 mm i.d.) and 70 μl·min^-1 for capillary (50 μm i.d.). Increasing the temperature up to 140 °C, together with temperature programming, markedly improves the separation and peak shapes within short analysis time. Sub-critical state is guaranteed.

Key words: Sub-critical water chromatography, FID, Polar compounds

Supporting:

Lu Feng, Li Ling, Sun Peng, Wu Yutian. Sub-critical Column and Capillary Chromatography with Water as Mobile Phase and Flame Ionization Detection[J]. .

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Sub-critical Column and Capillary Chromatography with Water as Mobile Phase and Flame Ionization Detection

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