Effects of Polygonum hydropiper on the expressions of inflammatory cytokines and cytochrome P450 enzymes in mice with E. coli-induced diarrhea

doi:10.5246/jcps.2022.08.052

Abstract

Abstract:

In the present study, we aimed to investigate the effects of Polygonum hydropiper aqueous extract (PHE) on the expression levels of inflammatory cytokines and cytochrome P450 (CYPs) in mice with E. coli-induced diarrhea. BALB/c mice were randomly divided into the control group, model group, enrofloxacin-treated group, and two PHE-treated groups with different doses. The diarrhea model was established by intraperitoneal injection of enteropathogenic E. coli (EPEC) in mice. The enrofloxacin-treated group was given enrofloxacin at 5 mg/kg by intragastric gavage (i.g.) for 11 d. PHE-treated groups were given PHE at 5 g/kg and 10 g/kg by i.g. for 11 d. The histopathological characteristics of the duodenum and liver were observed by HE staining. The levels of inflammatory cytokines and CYPs in the duodenum and liver of mice were determined by ELISA. The mRNA and protein expressions of CYPs were determined by qRT-PCR and Western blotting analysis, respectively. The results showed that PHE could significantly alleviate the injury of the duodenum and liver induced by EPEC infection, reduce the contents and mRNA expressions of inflammatory cytokines, and regulate the mRNA and protein expressions of the major subtypes of CYPs. These findings indicated that PHE had an apparent therapeutic effect on EPEC-induced diarrhea, and its mechanism might be related to inhibition of inflammatory cytokines and regulation of CYPs.

Key words: Polygonum hydropiper L., Enteropathogenic E. coli, Diarrhea, Inflammatory cytokines, Cytochrome P450

Supporting:

Jiahuan Huang, Ling Yue, Mingru Zhang, Quan Yang, Xuanxuan Cheng. Effects of Polygonum hydropiper on the expressions of inflammatory cytokines and cytochrome P450 enzymes in mice with E. coli-induced diarrhea[J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(8): 622-633.

Figures/Tables 8

Table 1. Primer sequences.

Table 2. Contents of TNF-α, IL-1β, IL-6, and LIF in duodenum and liver of mice.

Table 3. Contents of CYPs in duodenum and liver of experimental mice.

Table 4. Relative mRNA expression levels of TNF-α, IL-1β, IL-6, and LIF in duodenum and liver of mice.

Table 5. Relative mRNA expressions of CYPs in the duodenum of mice.

Table 6. Relative mRNA expressions of CYPs in the liver of mice.

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