http://jcps.bjmu.edu.cn

Journal of Chinese Pharmaceutical Sciences ›› 2022, Vol. 31 ›› Issue (5): 343-359.DOI: 10.5246/jcps.2022.05.030

• Original articles • Previous Articles     Next Articles

Network pharmacological analysis of Xiao-Xu-Ming decoction against ischemic stroke and verification of its mechanism of anti-inflammation and neurovascular protection in vivo

Yinglin Yang1,2, Shanshan Zhang1,2, Man Liu1,2, Dongni Liu1,2, Yuehua Wang1,2,*(), Guanhua Du1,2,*()   

  1. 1 State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
    2 Beijing Key Laboratory of Drug Target Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
  • Received:2021-12-12 Revised:2022-02-18 Accepted:2022-03-06 Online:2022-06-02 Published:2022-06-02
  • Contact: Yuehua Wang, Guanhua Du

Abstract:

Stroke is a major cause of severe disability and death. Xiao-Xu-Ming decoction (XXMD) is an effective prescription for stroke and its sequelae, while its effective ingredients and mechanism are still unclear. In the present study, we aimed to explore the effective ingredients and mechanism of XXMD in treating cerebral ischemia using network pharmacology. The main chemical components and targets of 12 herbs of?XXMD were obtained by the TCMSP database and analysis platform database. The active components in XXMD were screened according to oral utilization and drug-like properties. Then, the cerebral ischemia targets were obtained through GeneCards, OMIM, TTD, Diligent and Drugbank databases. We analyzed the pathophysiological processes and pathways involved in the treatment of cerebral ischemia with XXMD by using the Metascape data analysis platform. Results showed that β-sitosterol, kaempferol, quercetin, stigmasterol, wogonin, and catechins might be the potential core active ingredients of XXMD in the treatment of cerebral ischemia. The therapeutic effect of XXMD on stroke was mainly exerted through regulating neuroinflammatory response and neurovascular protection. Furthermore, the anti-neuroinflammation and neurovascular protection of XXMD were further confirmed using?cerebral ischemia rats. Collectively, our findings revealed that the mechanism of XXMD on the treatment of cerebral ischemia was related to anti-neuroinflammation and neurovascular protection.

Key words: Xiao-Xu-Ming decoction, Cerebral ischemia, Network pharmacology, Neuroprotection

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