Determination of inulin-type fructo-oligosaccharides in inulin by HPLC-ELSD

doi:10.5246/jcps.2022.01.003

Abstract

Abstract:

To develop an HPLC-ELSD method for the determination of nine inulin-type fructo-oligosaccharides in inulin, the HPLC-ELSD system consisted of Waters XBridge^? Amide column (4.6 mm × 250 mm, 5 μm) with a gradient elution mobile phase consisting of acetonitrile and water at a flow rate of 1.2 mL/min at 30 oC. The detector was an Agilent Technologies 380-ELSD. The drift tube temperature for the ELSD was set at 55 oC with a nitrogen flow rate of 1.8 L/min. The injection volume was 15 μL. The results showed that the detection range for the nine inulin-type fructo-oligosaccharides was 3.81–30.60 μg R²= 0.99969 for kestose, 3.73–29.97 μg R²= 0.99981 for nystose, 3.82–30.69 μg R²= 0.99993 for fructosylnystose, 3.80–30.48 μg R²= 0.99995 for GF₅, 3.73–29.96 μg R²= 0.99993 for GF₆, 3.78–30.30 μg R²= 0.99983 for GF₇, 3.82–30 μg R²= 0.99989 for GF₈, 3.71–29.80 μg R²= 0.99974 for GF₉, 3.61–29.00 μg R²= 0.99970 for GF₁₀, respectively. The recovery of the nine oligosaccharides ranged between 96.48%–100.84% (n = 6). The method was simple, accurate, and reproducible that it could be used as an analytical method for evaluating the quality of inulin effectively.

Key words: Inulin, Inulin-type fructo-oligosaccharides, Quantitative determination

Supporting:

Xiaoyu Cui, Pan Wang, Yidian Mo, Xuyang Ding, Shizhong Chen, Hongzhu Guo, Qin Hu, Zhongjun Li. Determination of inulin-type fructo-oligosaccharides in inulin by HPLC-ELSD[J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(1): 23-30.

Figures/Tables 7

Table 1. Gradient elution table of inulin.

Table 2. Linear regression data and LOD and LOQ for inulin-type FOS GF2–GF10.

Table 3. Precision, stability, and repeatability for nine inulin-type FOS.

Table 4. Accuracy for inulin-type FOS GF2–GF10.

Table 5. Nine inulin-type FOS contents in two samples.

References 19

[1]	Coussement, P.A.A. Inulin and oligofructose: safe intakes and legal status. J. Nutr. 1999, 129, 8161–8170.
[2]	Husemann, T. Pharmacographia. A history of the principal drugs of vegetable origin, met with in great Britain and British India. By Friedrich A. Flückiger, phil. dr., professor in the university of strassburg, and Daniel hanbury, F. R. s., fellow of the Linnean and chemical societies of london. second edition. Macmillan and co. 1879.803 S. in octav. Arch. Pharm. Pharm. Med. Chem. 1880, 216, 235–238.
[3]	Bacon, J.S.D.; Edelman, J. The carbohydrates of the Jerusalem artichoke and other Compositae. Biochem. J. 1951, 48, 114–126.
[4]	Niness, K.R. Inulin and oligofructose: what are they? J. Nutr. 1999, 129, 1402S–1406S.
[5]	Carpita, N.C.; Kanabus, J.; Housley, T.L. Linkage structure of fructans and fructan oligomers from triticum aestivum and festuca arundinacea leaves. J. Plant Physiol. 1989, 134, 162–168.
[6]	Liu, F.; Prabhakar, M.; Ju, J.; Long, H.; Zhou, H.W. Effect of inulin-type fructans on blood lipid profile and glucose level: a systematic review and meta-analysis of randomized controlled trials. Eur. J. Clin. Nutr. 2017, 71, 9–20.
[7]	Lopez, H.W.; Coudray, C.; Levrat-Verny, M.A.; Feillet-Coudray, C.; Demigné, C.; Rémésy, C. Fructooligosaccharides enhance mineral apparent absorption and counteract the deleterious effects of phytic acid on mineral homeostasis in rats. J. Nutr. Biochem. 2000, 11, 500–508.
[8]	Cashman, K.D. A prebiotic substance persistently enhances intestinal calcium absorption and increases bone mineralization in young adolescents. Nutr. Rev. 2006, 64, 189–196.
[9]	Griffin, I.J.; Davila, P.M.; Abrams, S.A. Non-digestible oligosaccharides and calcium absorption in girls with adequate calcium intakes. Br. J. Nutr. 2002, 87, S187–S191.
[10]	Mazraeh, R.; Azizi-Soleiman, F.; Jazayeri, S.M.H.M.; Noori, S.M.A. Effect of inulin-type fructans in patients undergoing cancer treatments: a systematic review. Pak. J. Med. Sci. 2019, 35, 575–580.
[11]	Messaoudi, M.; Rozan, P.; Nejdi, A.; Hidalgo, S.; Desor, D. Behavioural and cognitive effects of oligofructose-enriched inulin in rats. Br. J. Nutr. 2005, 93, S27–S30.
[12]	Gupta, N.; Jangid, A.K.; Pooja, D.; Kulhari, H. Inulin: a novel and stretchy polysaccharide tool for biomedical and nutritional applications. Int. J. Biol. Macromol. 2019, 132, 852–863.
[13]	Marrubini, G.; Appelblad, P.; Maietta, M.; Papetti, A. Hydrophilic interaction chromatography in food matrices analysis: an updated review. Food Chem. 2018, 257, 53–66.
[14]	Zhang, Q.; Yang, F.Q.; Ge, L.Y.; Hu, Y.J.; Xia, Z.N. Recent applications of hydrophilic interaction liquid chromatography in pharmaceutical analysis. J. Sep. Sci. 2017, 40, 49–80.
[15]	Ikegami, T. Hydrophilic interaction chromatography for the analysis of biopharmaceutical drugs and therapeutic peptides: a review based on the separation characteristics of the hydrophilic interaction chromatography phases. J. Sep. Sci. 2019, 42, 130–213.
[16]	Yang, Z.M.; Hu, J.; Zhao, M.Y. Isolation and quantitative determination of inulin-type oligosaccharides in roots of Morinda officinalis. Carbohydr. Polym. 2011, 83, 1997–2004.
[17]	Li, J.; Hu, D.J.; Zong, W.R.; Lv, G.; Zhao, J.; Li, S.P. Determination of inulin-type fructooligosaccharides in edible plants by high-performance liquid chromatography with charged aerosol detector. J. Agric. Food Chem. 2014, 62, 7707–7713.
[18]	Nyangale, E.P.; Farmer, S.; Keller, D.; Chernoff, D.; Gibson, G.R. Effect of prebiotics on the fecal microbiota of elderly volunteers after dietary supplementation of Bacillus coagulans GBI-30, 6086. Anaerobe. 2014, 30, 75–81.
[19]	Chi, L.D.; Khan, I.; Lin, Z.B.; Zhang, J.W.; Lee, M.Y.S.; Leong, W.; Hsiao, W.L.W.; Zheng, Y. Fructo-oligosaccharides from Morinda officinalis remodeled gut microbiota and alleviated depression features in a stress rat model. Phytomedicine. 2020, 67, 153157.