Application of L-Cys-Capped CdS Nanoparticles in Determination of Nucleic Acid by Resonance Light-Scattering Technique

Abstract

Abstract: Aim To determine nucleic acid (DNA) using Nanometer-sized L-cysteine-capped CdS particles by resonance light scattering (RLS) method. Methods The nano-particles synthesized by a colloidal aqueous method were water-soluble, stable, and highly luminescent. The RLS of L-Cys-CdS particles were greatly quenched by DNA in Tris-HCl solutions. The intensity of RLS at 344 nm was proportional to the concentration of DNA. Results The linearity range of the calibration curve was 0.01-1.0 μg·mL^-1 for calf thymus DNA and 0.04-1.5 μg·mL^-1 for salmon sperm DNA. The detection limits (3 δ) were 8 ng·mL^-1 for calf thymus DNA and 10 ng·mL^-1 for salmon sperm DNA. Conclusion This method is simple, sensitive, and capable of avoiding the use of toxic dyes.

Key words: CdS, CdS, resonance light scattering, resonance light scattering, cysteine, cysteine, DNA, DNA

CLC Number:

R917.02

R917.797

Supporting:

DAI Mei-ling, YAN Zheng-yu^*, QU Ping, SHAO Xiu-fen. Application of L-Cys-Capped CdS Nanoparticles in Determination of Nucleic Acid by Resonance Light-Scattering Technique[J]. .

References

[1] Holden M, Pirie NW. Treatments affecting the ultraviolet absorption spectrum of ribonucleic acid from three sources [J]. Biochem Biophys Acta, 1955, 16 (3) : 317-321.
[2] Gendimenico GJ , Bouquin PL, Tramposch KM. Diphenylamine-colorimetric method for DNA assay: a shortened procedure by incubating samples at 50 degrees C [J]. Anal Biochem , 1988, 173 (1) : 45-48.
[3] LePecq JB, Paoletti C. A new fluorometric method for RNA and DNA determination [J]. Anal Biochem, 1966, 17 (1) : 100-107.
[4] Rago R, Mitchen J , Wilding G. DNA fluorometric assay in 96-well tissue culture plates using Hoechst 33258 after cell lysis by freezing in distilled water [J]. Anal Biochem, 1990, 191 (1) : 31-34.
[5] Rye HS, Dabora JM, Quesada MA, et al. Fluorometric assay using dimeric dyes for double and single-stranded DNA and RNA with picogram sensitivity [J]. Anal Biochem , 1993, 208 (1) : 144-150.
[6] Micali N, Mallamace F, Castriciano M, et al. Separation of scattering and absorption contributions in UV/visible spectra of resonant systems [J]. Anal Chem , 2001, 73 (20) : 4958-4963.
[8] Zhu CQ, Wu YQ, Zheng H, et al. Determination of nucleic acids by near-infrared fluorescence quenching of hydrophobic thiacyanine dye in the presence of Triton X-100 [J]. Anal Sci, 2004, 20 (6) : 945-949.
[9] Zhang G, Shuang S, Dong C, et al. Investigation on DNA assembly to neutral red-cyclodextrin comp lex bymolecular spectroscopy [J]. J Photochem Photobiol B, 2004, 74 (2-3) : 127-134.
[10] Chen Z, DingW, L iang Y, et al. Determination of nucleic acids based on their resonance light scattering enhancement effect on metalloporphyrin derivatives [J]. Microchim Acta, 2005, 150 (1) : 35-42.
[11] Chen F, Huang J, Ai X, et al. Determination of DNA by Rayleigh light scattering enhancement of molecular “light switches” [J]. Analyst, 2003, 128 (12) : 1462-1466.
[12] Liu R, Yang J , Wu X, et al. Resonance double light scattering method for the determination of proteins with morin2CTMAB [J]. Spectrochim Acta A , 2002, 58 (14) : 3077-3083.
[13] Wang M, Yang J, Wu X, et al. Study of the interaction of nucleic acids with acridine red and CTMAB by a resonance light scattering technique and determination of nucleic acids at nanogram levels [J]. Anal Chim Acta, 2000, 422 (3) : 151-158.
[14] Hossain Z, Huq F. Studies on the interaction between Ag⁺ and DNA [J]. J Inorg Biochem , 2002, 90 (1) : 85-96.

[1]	Yingyuan Lu, Mei Zhang, Shengju Yin, Xiaona Dong, Zhiyuan Zhang, Haixu Cheng, Pengfei Tu, Guifang Dou, Yongsheng Che, Zhenghui Xu, Feng Xu, Xian Wang, Chuang Lu, Yaqing Lou, Guoliang Zhang. Epigenetic variants of xenobiotic metabolism affect individual differences in antiepileptic drug 3,4-DCPB pharmacokinetic phenotype [J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(1): 1-16.
[2]	Yunong Pang, Yinwen Liang, Yugang Wang, Fan Lei, Zhiyi Yuan, Dongming Xing, Jun Li, Lijun Du. Effect of berberine against cerebral ischemia and reperfusion involving in the methylation of PPARγ promoter [J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(3): 170-182.
[3]	Yi Yang, Fan Yi, Pei Zhang, Hua Li, Quan Du. Dnmt1 regulates adipogenesis by Cdkn1a methylation [J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(7): 488-495.
[4]	Pengyu Li, Zhiwen Li, Qi Wang, Junlin He. Studies on the combinatorial modifications with two amino groups on the catalytic core of 10-23 DNAzyme [J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(12): 851-857.
[5]	Wenfang Huang, Cangman Zhang, Sensen Li, Yi Liang, Hong Wang, Shizhong Chen. On-line HPLC-DAD coupled with ESI-IT-TOF-MS and fluorescence detection to identify DNA-binding compounds from Lithospermum erythrorhizon using acridine orange as the fluorescence probe [J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(9): 581-590.
[6]	Manman Gu, Yanfei He, Chunyang Han, Qi Zhou, Tengfei Liu, Yanfei Huang, Cuiyan Liu. Cytotoxicity effects of Rhizoma Coptidis on L929 murine fibroblast cells [J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(2): 111-120.
[7]	Mingming Chu, Xia Yuan, Xuan Jia, Ting Sun, Wei Guo, Xiao Jiang, Jingtao Liu, Runtao Li, Jingrong Cui. DNA damaging effect of SLXM-2, a derivative of cyclophosphamide, on hepatocarcinoma H₂₂ cells in vivo [J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(1): 16-21.
[8]	Qing Yang, Ji-Rang Zhu, Qi Sun^, Jing-Rong Cui, Run-Tao Li, Ze-Mei Ge^ . A novel class of cyclophosphamide prodrug: structure-activity relationships of cyclophosphamide piperaziniums [J]. , 2010, 19(1): 24-33.
[9]	Wei-Wei He, Zhao-Fei Liu, Dan Liu, Bing Jia, Fan Wang^, Yu-Xin Cui^*. Preliminary in vitro biological evaluation of novel O⁶-benzylguanine derivative-precursors of PET tracers for the DNA repair protein AGT [J]. , 2008, 17(1): 70-74.
[10]	Lu-Jun Wang, Peng-Fei Yue, Yan-Ling Zhao, Pei-Lie Cai, Xu Zhou, Hai-Long Yuan^* . Preparation and study of anti-hepatitis B virus activity in vitro of oxymatrine phospholipid complex [J]. , 2007, 16(2): 146-152.
[11]	Jun Jiang, Xiao-Da Yang^*, Kui Wang . Inhibition of cysteine protease papain by metal ions and polysulfide complexes,especially mercuric ion [J]. , 2007, 16(1): 1-8.
[12]	GUO Ming^*, TAN Fei, HU Run-huai, YU Qing-sen. Interaction between Mitomycin C and DNA at Paraffined Graphite Electrode [J]. , 2005, 14(4): 199-203.
[13]	HUANG Wen-zhe, DONG Ting-xia, QI Huan-yang, LU Zhen-qiang, ZHAN Hua-qiang^. Identification of Stellaria media* by PCR [J]. , 2005, 14(3): 144-148.
[14]	QI Rong, PING Qi-neng^*, XU Rui-yang, SHI Yong-ping. Effect of Chitosan-Cysteine Conjugate on Enzymatic Degradation and Hypoglycemic effect of Insulin [J]. , 2004, 13(2): 124-129.
[15]	Xu Zhidong, Liu He, Wang Min, Xiao Sulong, Yang Ming^*, Bu Xianhe . Cobalt (II) Complex of 6,7-Dicycanodipyridoquinoxaline with Antitumor Activities: Synthesis, Crystal Structure and Binding with DNA [J]. , 2002, 11(4): 125-131.