Optimization of ultrasound assisted extraction of six major compounds from Cimicifugae Rhizome

doi:10.5246/jcps.2016.05.042

Abstract

Abstract:

Cimicifugae Rhizome (RC) is one of many commonly used traditional Chinese medicines. This study used Box-Behnken Design (BBD), a widely used form of response surface methodology (RSM), to optimize the conditions of the ultrasound-assisted extraction (UAE) for the extraction of phenolic compounds from CimicifugaeRhizome. The influence of three independent variables,including ethanol concentration (%), extraction time (min) and solvent-to-material ratio (mL/g), on the extraction yields of phenolic compounds were examined. The results demonstrated that the optimal ultrasound-assisted extraction (UAE) conditions were as follows: ethanol concentration of 68%, an extraction time of 19 min, and solvent-to-material ratio of 30 mL/g for the extraction of caffeic acid, cimicifugoside, ferulic acid, isoferulicacid, cimifugin, 4'-O-β-glucopyranosyl-5-O-methylvisamminol. These results demonstrated the suitability of UAE for the extraction of phenolic compounds from CimicifugaeRhizome.

Key words: Ultrasound assisted extraction, Response surface methodology, CimicifugaeRhizome, HPLC

CLC Number:

R284

Supporting: Suzhou Youth science and technology plan (Grant No. KJXW2015028).

Wei Chen, Qin Yuan, Junping Cheng, Huinan Ge, Guorong Jiang, Rong Chen. Optimization of ultrasound assisted extraction of six major compounds from Cimicifugae Rhizome[J]. Journal of Chinese Pharmaceutical Sciences, 2016, 25(5): 373-379.

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