Rapid screening of anti-HIV ingredients in Artemisia rupestris L. extracts interacting with V3 loop region of HIV-1 gp120 and reverse transcriptase by affinity capillary electrophoresis and capillary zone electrophoresis

doi:10.5246/jcps.2015.09.079

Journal of Chinese Pharmaceutical Sciences ›› 2015, Vol. 24 ›› Issue (9): 625-629.DOI: 10.5246/jcps.2015.09.079

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Rapid screening of anti-HIV ingredients in Artemisia rupestris L. extracts interacting with V3 loop region of HIV-1 gp120 and reverse transcriptase by affinity capillary electrophoresis and capillary zone electrophoresis

Yiran Zhao¹, Zhongjie Li¹, Yong Jiang², Xiaodan Zhang¹, Xiaomei Ling^1*

1. Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
2. Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2015-05-18 Revised:2015-06-20 Online:2015-09-18 Published:2015-06-24
Contact: Tel.: 86-10-82801590, E-mail: lingxm@bjmu.edu.cn
Supported by:
National Natural Science Foundation (Grant No. 81373372) and Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20110001110021 and 20130001110059).

Abstract

Abstract:

HIV-1 gains entry into target cells by sequentially interacting with cellular receptors and co-receptors. Both the receptorand co-receptor are recognized by HIV-1 envelope protein gp120, which plays a key role in the entry process of HIV-1 into cells. The development of new inhibitors is essential since the viral enzyme reverse transcriptase (RT) is one of the first targets of antiretroviral therapy. It has been reported that a variety of natural plants, such as Artemisia rupestris L., have anti-viral pharmacological activity, and they might be the potential inhibitors of RT or V3 loop of gp120 against HIV-1. RIQRGPGRAFVTIGK (R15K), the relatively conserved region of V3 loop, can be used for binding research. In this work, we analyzed the interactions between different extracts from Artemisia rupestris L. and R15K by affinity capillary electrophoresis (ACE). Moreover, we analyzed the interactions betweendifferent extracts from Artemisia rupestris L. and RT by capillary zone electrophoresis (CZE). Our data showed that the chloroform extract of Artemisia rupestris L. was active among the different plant extracts, which was consistent with previous studies. Taken together, our study provided a rapid screening method to seek anti-HIV ingredients in natural plants’ extracts.

Key words: HIV, V3 loop, RIQRGPGRAFVTIGK, Reverse transcriptase, Affinity capillary electrophoresis, Capillary zone electrophoresis

CLC Number:

R917

Supporting:

Yiran Zhao, Zhongjie Li, Yong Jiang, Xiaodan Zhang, Xiaomei Ling . Rapid screening of anti-HIV ingredients in Artemisia rupestris L. extracts interacting with V3 loop region of HIV-1 gp120 and reverse transcriptase by affinity capillary electrophoresis and capillary zone electrophoresis[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(9): 625-629.

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Rapid screening of anti-HIV ingredients in Artemisia rupestris L. extracts interacting with V3 loop region of HIV-1 gp120 and reverse transcriptase by affinity capillary electrophoresis and capillary zone electrophoresis

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