Simultaneous determination of seven principal constituents in Asari Radix et Rhizoma by HPLC

doi:10.5246/jcps.2015.08.067

Abstract

Abstract:

A method using high-performance liquid chromatography for the simultaneous determination of seven principal constituentsincluding xanthoxylol, kakuol, methyleugenol, l-sesamin, safrole, sarisan, and l-asarinin in Asari Radix et Rhizoma (Xixin in Chinese) was developed. Fifty five samples of Asari Radix et Rhizoma from different sources (35 samples from Asarum heterotropoides var. mandshuricum, 3 samples from Asarum sieboldii var. seoulense, and 17 samples from Asarum sieboldii) were analyzed with this method. Chromatographic separation was achieved on an Agilent Zorbax SB-C₁₈ column (4.6 mm×25 cm, 5 μm) using a gradient elution with mobile phase of aqueous (A) and acetonitrile (B). The assay was carried out at 20 °C and with detection at 287 nm. All calibration curves showed good linearity (r²>0.999) within the tested ranges. The average recoveries were in the range of 96.0%-103.1% with relative standard deviation (RSD) less than 2.54%. The developed method was accurate with high sensitivity and good reproducibility. The content of volatile methyleugenol in LiaoXixin(the root and rhizome of A. heterotropoides var. mandshuricum and A. sieboldii var. seoulense) was (6.17±5.13) mg/g, which was higher than (0.58±0.40) mg/g in HuaXixin (the root and rhizome of A. sieboldii). The toxic safrole in LiaoXixin was (2.66±2.16) mg/g, which was lower than (6.44±3.89) mg/g in HuaXixin. This is the first report on the simultaneous and quantitative determination of anthoxylol and sarisan in Asari Radix et Rhizoma.

Key words: Asari Radix et Rhizoma, HPLC-DAD, determination, Anthoxylol, Sarisan

CLC Number:

R284

Supporting:

Chen Cao, Jingzhe Wang, Lu Wang, Mingying Shang, Guangxue Liu, Feng Xu, Xuan Wang, Shaoqing Cai . Simultaneous determination of seven principal constituents in Asari Radix et Rhizoma by HPLC[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(8): 530-537.

References

[1] The State Pharmacopoeia Commission of People’s Republic of China, Pharmacopoeia of the People’s Republic of China, Vol.1. Beijing: China Medical Science and Technology Press. 2010, 214.

[2] Xie, W.; Lu, M.W. Chin. Pharmacol. Bull. 1993, 9, 389.

[3] Xiong, Y.L.; Jing, Y.; Shang, M.Y.; Li, C.L.; Ye, J.; Wang, X.; Cai, S.Q. Chin. J. Chin. Mater. Med. 2009, 34, 2252-2257.

[4] Shi, H.X.; Jia, B.; Han, L.; Shen, T.; Peng, C.; You, F.M.; Liu, X.L. Fujian J. TCM. 2009, 40, 42-45.

[5] Zhang, Y.; Song, Z.Y.; Wang, L.L. China Pharm. 2007, 16, 62-63.

[6] Song, L.; Zhao, R.G.T.; Que, L.; Wan, C.P.; Tang, Z.G. Chin. J. Ethnomed. Ethnopharm. 2008, 4, 50-51.

[7] Li, X.G.; Zheng, Y.L.; Zhang, C.X. Acta Agric. Univ. Jilinesis. 1988, 10, 28-33.

[8] Yu, J.; Ma, C.M.; Long, C.F.; Wang, X.; Shang, M.Y.; Cai, S.Q.; Hattori, M.S.; Namba, T.N. J. Chin. Pharm. Sci. 2009, 18, 183-185.

[9] Wang, B.B.; Qi, W.; Wang, L.L; Kong, D.Q.; Kano, Y.H.; Li, J.X.; Yuan, D. J. Chin. Pharm. Sci. 2014, 24, 480-489.

[10] Han, J.Y.; Sun, C.L.; Ji, M.S. Chin. Agric. Sci. Bull. 2011, 27, 46-50.

[11] National Toxicology Program. Natl. Toxicol. Program. Tech. Rep. Ser. 2000, 491, 411-412.

[12] Li, R.S.; Zhou, W.M.; Li, X.K.; Zou, Y. J. Northwest A & F Univ.(Nat. Sci. Ed.) 2007, 35, 232-234.

[13] Xie, W.; Lu, M.W. Ningxia Med. J. 1995, 17, 121-124.

[14] Zhang, L. M.D. thesis, Shenyang Pharmaceutical University. 2008.

[15] Hashimoto, K.; Yanagisawa, T.; Okui, Y.; Ikeya, Y.; Maruno, M.; Fujita, T. Planta Med. 1994, 60, 124-127.

[16] Fan, S.L.; Liu, L.P.; Bian, K.J. Chin. J. Mod. Appl. Pharm. 2010, 27, 908.

[17] Wang, D.; Wang, X.J.; Xia, X.H. Chin. J. Chromatogr. 1997, 15, 85.

[18] Wu, Z.G.; Wang, Q.W.; Liu, X.Y.; Li, J.C.; Qian, X.H.; Lu, L.H.; Wang, Q.F.; Li, X.H. J. Fourth. Mil. Med. Univ. 2008, 29, 1175.

[19] Wang, G. F.; Zhang, S.Y.; Zhu, S.Q. Drug Anal. 2001, 19, 251-253.

[20] Guo, Z.J.; Liu, H.; Wang, L. Chin. Med. , 24, 273-274.2001

[21] Zhang, L.; Chen, X.H.; Liu, Y.L.; Zhang, J.; Bi, K.S. China Tradit. Herb. Drugs. 2008, 39, 1098-1100.

[22] Liu, Y.H.; Yi, J.H.; Huang, J.; Huang, Z.F.; Liu, Y.H. Chin. J. Pharm. Anal. 2012, 32, 822-825.