Evaluation of the physicochemical properties and antioxidant activities of polysaccharides from Semen Euryales

doi:10.5246/jcps.2014.08.075

Abstract

Abstract:

Polysaccharides were isolated from Semen Euryales by water extraction, alcohol precipitation and deproteinization. A refined polysaccharide, named EPJ, was separated into nine fractions by DEAE-52 cellulose chromatography and one of them was separated into two fractions by Sephadex G-100 column. The molecular weight of EPJ was determined to 15 367 Da and the monosaccharide composition was mainly glucose and rhamnose with the molar ratio of 5.46:1. The FT-IR spectra showed carbohydrate-related absorbance of polysaccharide functional groups. In vitro assay showed EPJ could significantly scavenge DPPH radical, superoxide anion, hydrogen peroxide and exhibited a strong reducing power. In vivo assay indicated that EPJ was effective in a D-galactose induced aging mice model. Results showed EPJ significantly inhibited formation of MDA and increased the activities of SOD, CAT, GSH-Px in a dose-dependent manner in mice tissues. The findings obtained from this study indicated that polysaccharides from Semen Euryales could be explored for a wide prospect of benefit.

Key words: Semen Euryales, Polysaccharide, Physicochemical properties, Antioxidant activities

CLC Number:

R284

Supporting:

Rong Chen, Man Xue, Wei Chen, Qinan Wu. Evaluation of the physicochemical properties and antioxidant activities of polysaccharides from Semen Euryales[J]. Journal of Chinese Pharmaceutical Sciences, DOI: 10.5246/jcps.2014.08.075.

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