A high performance liquid chromatography method for the quantitative determination of ribavirin in human plasma and its application in a pharmacokinetics study

doi:10.5246/jcps.2013.04.052

Abstract

Abstract:

The clinical pharmacokinetics of ribavirin after a single oral dose of 600 mg ribavirin tablets in healthy Chinese volunteers was studied. A rapid and simple high performance liquid chromatography (HPLC) method was developed to determine the ribavirin concentration in human plasma. C18 column was used for separation with a column temperature of 25 °C, the mobile phase was ultrapure water adjusted to pH 3 with acetic acid at the flow rate of 1 mL/min, and the detection wavelength was set at 207 nm. The linear range of the standard curves was 50.4-2016.0 ng/mL and the lower limit of quantification (LLOQ) was 50.4 ng/mL. The relative recoveries of ribavirin were more than 90% in plasma. The RSD of the intra-day precision was less than 10% and that of inter-day was less than 15%. The pharmacokinetic parameters of ribavirin were calculated by WinNonlin. Results indicated that the two-compartment model was a better model for describing the pharmacokinetics profile of ribavirin than one-compartment model. The AUC0-t was 10807.8 h∙ng/mL, the CL/F was 64879.5 mL, and the Cmax was 525.1 ng/mL. These results provided the experimental data for the development of ribavirin dosage form.

Key words: Ribavirin, HPLC, Pharmacokinetics, Compartmental model

CLC Number:

R917.1

Supporting:

Hua Zhang, Guiling Wang, Kexin Li, Xuan Zhang^*, Qiang Zhang. A high performance liquid chromatography method for the quantitative
determination of ribavirin in human plasma and its application in a pharmacokinetics study[J]. , DOI: 10.5246/jcps.2013.04.052.

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A high performance liquid chromatography method for the quantitative
determination of ribavirin in human plasma and its application in a pharmacokinetics study

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