Quantification of the active ingredient quercetin of “Guangdong Wang-bu-liu-xing” by high-performance liquid chromatography with UV detection

Abstract

Abstract:

High-performance liquid chromatography with UV detection was used for the quantification of the flavonoid quercetin, the active compound found in “Guangdong Wang-bu-liu-xing”. The method was developed and demonstrated to provide superior performance over other commonly documented methods. This HPLC assay achieved high specificity through the use of a reversed-phase C18 column eluted with a mobile phase of methanol-0.4% (v/v) phosphoric acid (50:50, v/v) over the course of 30 min. UV detection at 360 nm was used. This analytical method provided excellent precision and a mean recovery of 99%, demonstrated by repeated analysis of 11 sample groups. Because of its high performance and simplicity, this HPLC assay can be readily utilized as a practical method for the quality control of active compounds extracted from Guangdong Wang-bu-liu-xing.

Key words: Guangdong Wang-bu-liu-xing, Quantification, HPLC, Quercetin

CLC Number:

R917.7

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Song Zeng, Xiuqi Han, Shuyuan Li^* . Quantification of the active ingredient quercetin of “Guangdong Wang-bu-liu-xing” by high-performance liquid chromatography with UV detection[J]. .

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http://jcps.bjmu.edu.cn/EN/Y2013/V22/I1/101

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