Antioxidantive activities and gastrodin determination of extracts of endophytic fungi isolated from Gastrodia elata Bl.

doi:10.5246/jcps.2020.03.018

Journal of Chinese Pharmaceutical Sciences ›› 2020, Vol. 29 ›› Issue (3): 206-213.DOI: 10.5246/jcps.2020.03.018

• Original articles • Previous Articles Next Articles

Antioxidantive activities and gastrodin determination of extracts of endophytic fungi isolated from Gastrodia elata Bl.

Xiaoxia Liang^#,*, Cheng Xiong^#, Yingying Gao, Qiaojia Fan

Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2019-11-15 Revised:2019-12-04 Online:2020-03-30 Published:2020-01-08
Contact: Tel.: +86-28-86291470, E-mail: liangxiaoxia@sicau.edu.cn
Supported by:
The Foundation of Industrialization Demonstration and Promotion of Standardized Planting of Dominant Species of Gastrodia elata in Yingjing (General Service Station) and the Foundation of Sichuan Science and Technology Plan Project (Grant No. 2019YFS0170).

Abstract

Abstract:

Gastrodia elata Bl. is a traditional Chinese herbal medicine with special pharmacological effects on headache and numbness. However, its wild sources are now in short supply because of over-excavation. A total of 14 endophytic fungi were isolated from Gastrodia elata Bl collected from Yinjing, in which 10 strains were identified, including Neonectria, Ascomycota, Fusarium, Oidiodendron and Dothiorella. All crude extracts showed some degrees of antioxidant activities by T-AOC method and DPPH methods, especially those from Oidiodendron GeR6 and Ascomycota sp. GeR2, which might be attributed due to their high phenolic contents. However, only Oidiodendron GeR6 contained gastrodin (39.2 μg/g) as the host plant in the crude extracts. In conclusion, the notable activities of GeR6 indicated its medical use as a natural antioxidant agent, as well as substitutable use for Gastrodia elata Bl. in the future.

Key words: Gastrodia elata Bl., Endophytic fungi, Antioxidantive activity, Gastrodin

CLC Number:

R284

Supporting:

The DNA sequence of endophytic fungi isolated from Gastrodia elata Bl.

Xiaoxia Liang, Cheng Xiong, Yingying Gao, Qiaojia Fan. Antioxidantive activities and gastrodin determination of extracts of endophytic fungi isolated from Gastrodia elata Bl.[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(3): 206-213.

References

[1] Wu, Z.Z.; Li, Y.S.; Liu, L.D.; Li, L.X.; Yang, Y. Study on the extraction technology and pharmacological function of active ingredient of Gastrodia elata Blume. Storage and Process. 2014, 14, 58-61.

[2] Liu, M.Q.; Luo, J.L. Research on extraction and purification of Gastrodia elata polysaccharides. China Pharm. 2011, 14, 1593-1596.

[3] Liyanapathirana, C.; Shahidi, F. Optimization of extraction of phenolic compounds from wheat using response surface methodology. Food Chem. 2005, 93, 47-56.

[4] Wang, L.; Zhang, X.G.; Li, X.Q. Studies on the main active ingredients and structure modification of Gastrodia elata. Hubei Agric. Sci. 2010, 5, 1146-1149.

[5] Luo, Z. .; Wu, J.Q.; Wu, M.C. The Antioxidative and antiviral activities of plant polysaccharide. J. Hubei Inst. Nat. 2007, 1, 77-81.

[6] Wang, L.P.; Zhou, S.M.; Dai, D.L.; Cao,J.S. Progress in plant phenolic compounds. Acta Agric. Zhejiangensis. 2010, 5, 696-701.

[7] Saikkonen, K.; Faeth, S.H.; Helander, M.; Sullivan, T.J. FUNGAL ENDOPHYTES: A continuum of interactions with host plants. Annu. Rev. Ecol. Syst. 1998, 29, 319-343.

[8] Stierle, A.; Strobel, G.; Stierle, D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science. 1993, 260, 214-216.

[9] Devari, S.; Jaglan, S.; Kumar, M.; Deshidi, R.; Guru, S.; Bhushan, S.; Kushwaha, M.; Gupta, A.P.; Gandhi, S.G.; Sharma, J.P.; Taneja, S.C.; Vishwakarma, R.A.; Shah, B.A. Capsaicin production by Alternaria alternata, an endophytic fungus from Capsicum annum; LC-ESI-MS/MS analysis. Phytochemistry. 2014, 98, 183-189.

[10] Chithra, S.; Jasim, B.; Sachidanandan, P.; Jyothis, M.; Radhakrishnan, E.K. Piperine production by endophytic fungus Colletotrichum gloeosporioides isolated from Piper nigrum. Phytomedicine. 2014, 21, 534-540.

[11] Shweta, S.; Gurumurthy, B.R.; Ravikanth, G.; Ramanan, U.S.; Shivanna, M.B. Endophytic fungi from Miquelia dentata Bedd., produce the anti-cancer alkaloid, camptothecine. Phytomedicine. 2013, 20, 337-342.

[12] Mo, L.; Kang, J.C.; He, J.; Cao, J.J.; Su, H. A preliminary Study of the Composition of Endophytic Fungi from Gastrodia elata. J. Fungal Res. 2009, 6, 211-215.

[13] Tang, X.J.; Xing, G.S.; Li, F.; Ren, N. Symbiotic germination between gastrodia elata and fungal species of mycena. Mycosystema. 2001, 2001, 137-141.

[14] Fisher, P.J.; Petrini, O.; Sutton, B.C. A comparative study of fungal endophytes in leaves, xylem and bark of Eucalyptus in Australia and England. Sydowia. 1993, 45, 338-345.

[15] Taga, M.S.; Miller, E.E.; Pratt, D.E. Chia seeds as a source of natural lipid antioxidants. J. Am. Oil Chem. Soc. 1984, 61, 928-931.

[16] Li, Y.L.; Xin, X.M.; Chang, Z.Y.; Shi, R.J.; Miao, Z.M.; Ding, J.; Hao, G.P. The endophytic fungi of Salvia miltiorrhiza Bge.f. alba are a potential source of natural antioxidants. Bot. Stud. 2015, 56, 5.

[17] Hamilton, C.E.; Gundel, P.E.; Helander, M.; Saikkonen, K. Endophytic mediation of reactive oxygen species and antioxidant activity in plants: a review. Fungal Divers. 2012, 54, 1-10.

[18] Govindappa, M.; Bharath, N.; Shruthi, H.B.; Santoyo, G. In vitro antioxidant activity and phytochemical screening of endophytic extracts of crotalaria pallida. Free. Radic. Antioxid. 2011, 1, 79-86.

[19] Murthy, N.K.; Pushpalatha, K.C.; Joshi, C.G. Antioxidant activity and phytochemical analysis of endophytic fungi isolated from Lobelia nicotianifolia. J. Chem. Pharm. Res. 2011, 3, 218-225.

[20] Sadananda, T.S.; Nirupama, R.; Chaithra, K.; Govindappa, M.; Chandrappa, C.P.; Raghavendra, B.V. Antimicrobial and antioxidant activities of endophytes from Tabebuia argentea and identification of anticancer agent (lapachol). J. Med. Plants Res. 2011, 5, 3643-3652.

Antioxidantive activities and gastrodin determination of extracts of endophytic fungi isolated from Gastrodia elata Bl.

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