Chemical constituents and analgesic activity of Aconitum kusnezoffii Reichb.

doi:10.5246/jcps.2018.12.087

Abstract

Abstract:

In the present study,we aimed to investigate the chemical constituents and analgesic activity of Aconitum kusnezoffii Reichb. The isolation and purification of components were achieved by a series of chromatography, including silica gel, Sephadex LH-20 and HPLC. By using spectroscopic analysis, their structures were identified. Using PDE-4A as analgesic target, moleculardocking was conducted between isolated compounds by using Schrodinger software. Neoline is a typical non-ester diterpene alkaloid. It was studied by using the mouse torsion body method and hot plate method. A total of 12 diterpene alkaloids were obtainedand identified as Mesaconitine(1), Bewutine (2), Bewudine (3), Songoramine (4), Songorine (5), Neoline (6), Talasamine (7), isotalatizidine (8), Hokbusine A (9), Mesaconine (10), 8-OEt-14-benzoylmesaconine (11), 8-Methoxy-14-benzoyl-beiwutinine (12).Compounds 9 and 12 were isolated from Aconitum kusnezoffii Reichb. for the first time. Twelve diterpenealkaloids could act on the analgesic target. Neoline is a typical non-ester diterpene alkaloid. It had significant analgesic effect. Diterpene alkaloids were the main components of Aconitum kusnezoffiiReichb., and they had good analgesic activity.

Key words: Aconitum kusnezoffii Reichb., Chemical constituents, Structural identification, Analgesic activity, Molecular docking

CLC Number:

R284

Supporting:

Qian Li, Shuding Sun, Meiying Wang, Caifeng Li, Dan Yuan, Hongzheng Fu. Chemical constituents and analgesic activity of Aconitum kusnezoffii Reichb.[J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(12): 855-863.

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