Preparation, optimization and in vitro evaluation of core-shell paclitaxel-loaded nanoparticles composed of MPEG-PLA and PLA

doi:10.5246/jcps.2015.02.012

Abstract

Abstract:

Nanoparticles with typical core-shell structure were prepared with a blend of methoxypoly(ethylene glycol)-poly(lactide)copolymer (MPEG-PLA) and poly (lactic acid) (PLA) along with paclitaxel by the O/W solvent evaporation method. An orthogonalexperiment L₉(3)³ was applied to get the best preparation conditions. The core-shell paclitaxel-loaded MPEG-PLA/PLA nanoparticleswith the highest drug loading efficiency were obtained when amount of MPEG-PLA, time of ultrasonication and volume of deionized water were 300 mg, 10 min and 30 mL, respectively. The release behavior of paclitaxel from the optimal MPEG-PLA/PLA nanoparticles showed that 22% of paclitaxel was released in 14 d. When incubating with human nasopharyngeal carcinoma cells expressing LMP1, these optimal nanoparticles showed a little lower tumor growth compared with free paclitaxel.

Key words: Core-shell nanoparticles, MPEG-PLA/PLA, Paclitaxel, Orthogonal design

CLC Number:

R943

Supporting:

Yu Sun, Xinming Zhang, Lin Tang. Preparation, optimization and in vitro evaluation of core-shell paclitaxel-loaded nanoparticles composed of MPEG-PLA and PLA[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(2): 104-110.

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