Combination of vitamin C and zinc gluconate prevented vanadium-induced tight junction leakage of MDCK cell monolayer

doi:10.5246/jcps.2013.05.058

Abstract

Abstract:

The tight junction disorder plays an important role in the pathological process of many chronic diseases, and is becoming a major concern for the clinical application of metal drugs, i.e. anti-diabetic vanadium compounds. The development of novel tight junction protecting agents has thus been a major research focus. Since oxidative stress is the primary cause for vanadium toxicity, the present work tested the protective effects of zinc gluconate (Zn²⁺) alone and when combined with vitamin C (VC) on the vanadium compound (VO(acac)₂)-mediated paracellular leakage of MDCK cells. The experimental results showed that VO(acac)₂ treatment significantly increased the paracellular permeability of MDCK monolayer. Zn²⁺ alone showed no protective effects and VC ameliorated tight junction leakage of MDCK cells when given in the basal chamber. Interestingly, unilateral treatment with the combination of Zn²⁺ and VC effectively prevented the increase of paracellular permeability. In addition, the combination of zinc and VC down-regulated the levels of reactive oxygen species in both the control and VO(acac)₂-treated MDCK cells and caused the elevation of intracellular Ca²⁺; both effects were beneficial for the maintenance of integrity of intercellular tight junction. Our results provided a simple but very effective method of preventing the metal toxicity for clinical application of anti-diabetic vanadium compounds.

Key words: Zinc gluconate, Vitamin C, Tight junction, Oxidative damage, Intracellular calcium

CLC Number:

R915

Supporting:

Zhihan Xu, Xinyi Wang, Ruyue Xiao, Xiaoda Yang^* . Combination of vitamin C and zinc gluconate prevented vanadium-induced tight junction leakage of MDCK cell monolayer[J]. , DOI: 10.5246/jcps.2013.05.058.

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