Chromatographic fingerprint analysis of Bupleuri Radix by HPLC-ELSD

doi:10.5246/jcps.2013.01.005

Abstract

Abstract:

To facilitate the species identification and quality assessment of Chaihu (Bupleuri Radix), a simple and valid chromatographic fingerprint method was developed. The method uses high-performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD) and the data analysis is assisted by professional analytical software recommended by the State Food and Drug Administration (SFDA). The results indicate that Nan Chaihu raw materials and Chaihu decoction pieces vary markedly in chemical quality, while Bei Chaihu raw materials are relatively more stable. Furthermore, it is obvious that Nan Chaihu is chemically very different from Bei Chaihu, suggesting that Nan Chaihu may not be suitable for medicinal use. In addition, the obvious differences between the chromatograms of decoction pieces and raw materials, especially the peaks between 30 and 40 min and after 45 min, indicate possible effects of the processing procedures on the chemicals. By analyzing the fingerprints of all samples, 12 main saponin-like fingerprint peaks, of which at least three are characteristic peaks of saikosaponins a, c, and d, are proposed to be considered for further characterization and quality evaluation of Chaihu.

Key words: Bupleuri Radix, Bupleurum chinense, Bupleurum scorzonerifolium, Chromatographic fingerprint, HPLC-ELSD, Saikosaponins

CLC Number:

R284.1

Supporting:

Yong Zhou, Yanliang Wang, Zhiqiao Ma, Bin Wang, Hong Liang, Yuying Zhao, Qingying Zhang^*. Chromatographic fingerprint analysis of Bupleuri Radix by HPLC-ELSD[J]. , DOI: 10.5246/jcps.2013.01.005.

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