Sensitive method for rapid estimation of Lornoxicam in bulk and its dosage form by RP-HPLC

doi:10.5246/jcps.2013.01.016

Abstract

Abstract:

Lornoxicam (6-chloro-4-hydroxy-2-methyl-N-2-pyridyl-2H-thieno-[2,3-e]-1,2-thiazine-3-carboxamide-1,1-dioxide) is persist as a non-steroidal anti-inflammatory drug of the oxicam class with analgesic, anti-inflammatory and antipyretic properties. A fast, accurate and sensitive chromatographic method for estimation of Lornoxicam was developed as no official method available for detection. The chromatographic separation employs isocratic elution by utilizing an inertsil ODS-C18, 250 mm×4.6 mm, 5 µm columns. Mobile phase consisting of solvent (40 mL acetonitrile and 60 mL 0.1 M phosphate buffer (pH 6.8 was adjusted with triethylamine)) endowed at a flow rate of 1.0 mL/min. The analyte was detected and quantified at 290 nm using UV detector. The method was validated according to ICH guidelines, illustrating to be accurate (recovery 99.08%-101.13%) and precise (intraday (0.27-1.32) and interday (0.59-1.59))within the corresponding linear range (10-60 µg/mL) with r² 0.9992.

Key words: Lornoxicam, RP-HPLC, Analgesic, Anti-inflammatory

CLC Number:

R917.7

Supporting:

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Prajapati Prajesh^*, Vaghela Vipul, Baraiya Dharmendra. Sensitive method for rapid estimation of Lornoxicam in bulk and its dosage form by RP-HPLC[J]. , DOI: 10.5246/jcps.2013.01.016.

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URL: http://jcps.bjmu.edu.cn/EN/10.5246/jcps.2013.01.016

http://jcps.bjmu.edu.cn/EN/Y2013/V22/I1/110

References

[1] Balfour, J.A.; Fitton, A.; Barradell, L.B. Drugs. 1996, 51, 639-657.

[2] Ghoneim, M.M.; Beltagi, A.M.; Radi, A. Anal. Sci. 2002, 18, 183-186.

[3] Ibrahim, C.; Nisa, K.; Sule, A. C.B.U.J. Sci. 2009, 5, 11-18.

[4] Prajesh, P.; Vipul, V.; Deepak, R.; Harshad, P.; Jasmin, K.; Dharmendra, B. J. Pharmaceut. Anal.

http://dx.doi.org/10.1016/j.jpha.2012.02.004

[5] Lakshmi, S.; Lakshmi, K.S; Tintu, T. Int. J. Pharm. Pharm. Sci. 2010, 2, 166-168.

[6] Waghmare, J.J. Int. J. PharmTech. Res. 2011, 3, 1603-1608.

[7] Rele, R.V.; Warkar, C.B. Inter. J. Pharm. Bio. Sci. 2011, 2, 124-129.

[8] Nemutlu, E.; Demircan, S.; Kir, S. Die Pharmazie. 2005, 60, 421-425.

[9] Young, H.K.; Hye, Y.J.; Eun-Seok, P. Arch Pharm. Res. 2007, 30, 905-910.

[10] Zeng, Y.L.; Chen, X.Y.; Zhang, Y.F. Acta Pharm. Sin. 2004, 39, 132-135.

[11] Radhofer-Welte, S.; Dittrich, P. J. Chromatogr. B. 1998, 707, 151-159.

[12] Attimarad, M. J. Basic Clin. Pharm. 2010, 1, 115-118.

[13] Akiko, N.; Nakashima, M.N.; Mitsuhiro, W. Bunseki Kagaku. 2005, 54, 755.

[14] Taha, E.A.; Salama, N.N.; Abdel Fattah, L.S. J. AOAC. Int. 2004, 87, 366-373.

[15] ICH. Text on Validation of Analytical Procedures: Methodology, International conference on Harmonization of Technical Requirements for Registration of Pharmaceutical for Human Use, Geneva: Jul 2000.

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