Quantitative determination of cyclophosphamide in rat plasma using an on-line SPE HPLC-DAD

doi:10.5246/jcps.2012.02.020

Abstract

Abstract:

A rapid and simple liquid chromatography method with on-line solid phase extraction was developed and validated for the quantitative determination of cyclophosphamide in rat plasma. The plasma sample was first extracted on an Acclaim^® Polar Advantage II C₁₈ guard column (PA II C₁₈, 10 mm×4.6 mm, 5 μm), which was also the on-line Extraction Cartridge SPE column, by washing with 100% H₂O for 1 min. The extracted sample was then eluted onto a PA II C₁₈ column (150 mm×4.6 mm, 5 μm) and separated by isocratic elution with acetonitrile–water (40:60, v/v). The mobile phase was run at a flow rate of 1.0 mL/min, and the UV detector was set at 195 nm. Retention time of cyclophosphamide was 4.3 min and the total run-time was 6 min. The linear range of the standard curve was from 1.0 to 200 μg/mL (r² = 0.9999), and the limits of quantification and detection were 1.0 μg/mL (RSD<10%, n = 5) and 0.3 μg/mL(RSD<13%, n = 5), respectively. Both intra- and inter-day variations were less than 5.6%. The developed method can be used for the therapeutic drug monitoring of cyclophosphamide in the clinic.

Key words: On-line SPE, HPLC-DAD, Cyclophosphamide, Rat plasma

CLC Number:

R917.1

Supporting:

Xiao-Na Li, Ning Yu, Jian-Mei Zhang, Wen-Si Lin, Xiao-Mei Ling^*, Ge Fu, Run-Tao Li, Jing-Rong Cui. Quantitative determination of cyclophosphamide in rat plasma using
an on-line SPE HPLC-DAD [J]. , DOI: 10.5246/jcps.2012.02.020.

References

[1] Baumann, F.; Preiss, R. J. Chromatogr. B. 2001, 764, 173-192.
[2] Kalhorn, T.F.; Ren, S.; Howald, W.N.; Lawrence, R.F.; Slattery, J.T. J. Chromatogr. B. 1999, 732, 287-298.
[3] Takamoto, S.; Sakura, N.; Namera, A.; Yashiki, M. J. Chromatogr. B. 2004, 806, 59-63.
[4] Delmas, A.; Gordien, J.B.; Bernadou, J.M.; Roudaut, M.; Gresser, A.; Malki, L.; Sauxa, M.C.; Breilh, D. J. Pharm. Biomed. Anal. 2009, 49, 1213-1220.
[5] Martins, I.; Souza, J.O.; Sanson, A.L.; Vieira, E.P. Lat. Am. J. Pharm. 2009, 28, 41-46.
[6] Bai, F.; Fraga, C.H.; Tagen, M.; Schaiquevich, P.; Hagedorn, N.; Stewart, C.F. J. Chromatogr. B. 2009, 877, 1709-1715.
[7] Swamy, G.C.; Errington, J.; Foot, A.; Boddy, A.V.; Veal, G.J.; Cole, M. Eur. J. Cancer. 2011, 47, 1556-1563.
[8] Ekhart, C.; Gebretensae, A.; Rosing, H.; Rodenhuis, S.; Beijnen, J.H.; Huitem, A.D. J. Chromatogr. B. 2007, 854, 345-349.
[9] Sadagopan, N.; Cohen, L.; Roberts, B.; Collard, W.; Omer, C. J. Chromatogr. B. 2001, 759, 277-284.
[10] Habib, A.D.; Boal, J.H.; Hilton, J.; Nguyen, T.; Chang, Y.H.; Ludeman, S.M. Biochem. Pharmacol. 1995, 50, 429-453.
[11] Bouligand, J.; Storme, T.; Laville, I.; Mercier, L.; Oberlin, O.; Lavillea, I.; Mercier, L.; Vassal, G.; Bourget, P.; Paci, A. J. Pharm. Biomed. Anal. 2005, 38, 180-185.
[12] Salgueiro, A.; Egea, M.A.; Valls, R.; Espina, M.; Garcı́a, M.L. J. Pharm. Biomed. Anal. 1999, 21, 611-618.
[13] Visser, N.F.C.; Lingeman, H.; Irth, H. J. Pharm. Biomed. Anal. 2003, 32, 295-309.
[14] Alnouti, Y.; Srinivasan, K.; Waddell, D.; Bi, H.B.; Kavetskaia, O.; Gusev, A. J. Chromatogr. A. 2005, 1080, 99-106.
[15] Hørning, O.B.; Theodorsen, S.; Vormb, O.; Jensen, O.N. J. Mass. Spectrom. 2007, 268, 147-157.
[16] Rozeta, E.; Morello, R.; Lecomte, F.; Martin, G.B.; Chiap, P.; Crommen, J.; Boos, K.S.; Hubert, P. J. Chromatogr. B. 2006, 844, 251-260.
[17] Zhu, X.; Sun,Y.X.; He X.J.; Qiu F.; Li, J.L.; Zhao, L.M. J. Chin. Pharm. Sci. 2011, 20, 70-76.

Quantitative determination of cyclophosphamide in rat plasma using
an on-line SPE HPLC-DAD

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